5pti
From Proteopedia
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| - | [[Image:5pti.gif|left|200px]] | + | [[Image:5pti.gif|left|200px]] |
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| - | '''STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT OF CRYSTAL FORM II''' | + | {{Structure |
| + | |PDB= 5pti |SIZE=350|CAPTION= <scene name='initialview01'>5pti</scene>, resolution 1.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT OF CRYSTAL FORM II''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 5PTI is a [ | + | 5PTI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTI OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of bovine pancreatic trypsin inhibitor. Results of joint neutron and X-ray refinement of crystal form II., Wlodawer A, Walter J, Huber R, Sjolin L, J Mol Biol. 1984 Dec 5;180(2):301-29. PMID:[http:// | + | Structure of bovine pancreatic trypsin inhibitor. Results of joint neutron and X-ray refinement of crystal form II., Wlodawer A, Walter J, Huber R, Sjolin L, J Mol Biol. 1984 Dec 5;180(2):301-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6210373 6210373] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: proteinase inhibitor (trypsin)]] | [[Category: proteinase inhibitor (trypsin)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:12:46 2008'' |
Revision as of 17:12, 20 March 2008
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| , resolution 1.0Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT OF CRYSTAL FORM II
Overview
The structure of form II crystals of bovine pancreatic trypsin inhibitor has been investigated by joint refinement of X-ray and neutron data. Crystallographic R factors for the final model were 0.200 for the X-ray data extending to 1 A resolution and 0.197 for the 1.8 A neutron data. This model was strongly restrained, with 0.020 A root-mean-square (r.m.s.) departure of bond lengths from their ideal values and 0.019 A r.m.s. departure of planar groups from planarity. The resulting structure was very similar to that of crystal form I (r.m.s. deviation for main chain atoms was 0.40 A); nevertheless larger deviations were observed in particular regions of the chain. Twenty out of 63 ordered water molecules occupy similar positions (deviation less than 1 A) in both models. Eleven amide hydrogens were found to be protected from exchange after three months of soaking the crystals in deuterated mother liquor at pH 8.2. Their locations were in excellent agreement with the results obtained by two-dimensional nuclear magnetic resonance, but the rates of exchange are much lower in the crystalline state.
About this Structure
5PTI is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure of bovine pancreatic trypsin inhibitor. Results of joint neutron and X-ray refinement of crystal form II., Wlodawer A, Walter J, Huber R, Sjolin L, J Mol Biol. 1984 Dec 5;180(2):301-29. PMID:6210373
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