6ebx
From Proteopedia
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| - | [[Image:6ebx.gif|left|200px]] | + | [[Image:6ebx.gif|left|200px]] |
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| - | '''STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION''' | + | {{Structure |
| + | |PDB= 6ebx |SIZE=350|CAPTION= <scene name='initialview01'>6ebx</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SCN:THIOCYANATE ION'>SCN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 6EBX is a [ | + | 6EBX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBX OCA]. |
==Reference== | ==Reference== | ||
| - | Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution., Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A, Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:[http:// | + | Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution., Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A, Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1418823 1418823] |
[[Category: Laticauda semifasciata]] | [[Category: Laticauda semifasciata]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:13:22 2008'' |
Revision as of 17:13, 20 March 2008
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| , resolution 1.7Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION
Overview
Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four disulfide bridges, was crystallized in a new orthorhombic form by using thiocyanate as crystallizing agent. The space group is P2(1)2(1)2(1) with a = 53.36 (4), b = 40.89 (4), c = 55.71 (5) A, V = 121533.1 A and Z = 8. X-ray diffraction data were recorded at the LURE synchrotron facility (lambda = 1.405 A). The structure was solved by molecular replacement and shows a dimeric association through an anti-parallel beta-sheet around the twofold non-crystallographic axis. The two independent molecules, one SCN- ion and 97 associated water molecules were refined by molecular dynamics and annealing techniques to R = 19.6% (10,913 Fobs, resolution 5-1.7 A). The thiocyanate ion is located at the interface of the dimer and close to the non-crystallographic twofold axis.
About this Structure
6EBX is a Single protein structure of sequence from Laticauda semifasciata. Full crystallographic information is available from OCA.
Reference
Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution., Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A, Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:1418823
Page seeded by OCA on Thu Mar 20 19:13:22 2008
