This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6fit

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:6fit.jpg|left|200px]]<br /><applet load="6fit" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:6fit.jpg|left|200px]]
-
caption="6fit, resolution 2.6&Aring;" />
+
 
-
'''FHIT-TRANSITION STATE ANALOG'''<br />
+
{{Structure
 +
|PDB= 6fit |SIZE=350|CAPTION= <scene name='initialview01'>6fit</scene>, resolution 2.6&Aring;
 +
|SITE= <scene name='pdbsite=AVE:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVE</scene> and <scene name='pdbsite=HNE:HIS+Triad+For+Which+This+Family+Was+Named'>HNE</scene>
 +
|LIGAND= <scene name='pdbligand=AMW:ADENOSINE MONOTUNGSTATE'>AMW</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Bis(5'-adenosyl)-triphosphatase Bis(5'-adenosyl)-triphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.29 3.6.1.29]
 +
|GENE= FHIT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
 +
}}
 +
 
 +
'''FHIT-TRANSITION STATE ANALOG'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
6FIT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=AMW:'>AMW</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Bis(5'-adenosyl)-triphosphatase Bis(5'-adenosyl)-triphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.29 3.6.1.29] Known structural/functional Sites: <scene name='pdbsite=AVE:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVE</scene> and <scene name='pdbsite=HNE:HIS+Triad+For+Which+This+Family+Was+Named'>HNE</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIT OCA].
+
6FIT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIT OCA].
==Reference==
==Reference==
-
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9323207 9323207]
+
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9323207 9323207]
[[Category: Bis(5'-adenosyl)-triphosphatase]]
[[Category: Bis(5'-adenosyl)-triphosphatase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
Line 27: Line 36:
[[Category: putative tumor suppressor]]
[[Category: putative tumor suppressor]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:33 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:13:26 2008''

Revision as of 17:13, 20 March 2008

Image:6fit.jpg


PDB ID 6fit

Drag the structure with the mouse to rotate
, resolution 2.6Å
Sites: and
Ligands:
Gene: FHIT (Homo sapiens)
Activity: Bis(5'-adenosyl)-triphosphatase, with EC number 3.6.1.29
Coordinates: save as pdb, mmCIF, xml



FHIT-TRANSITION STATE ANALOG


Overview

The histidine triad (HIT) protein family is among the most ubiquitous and highly conserved in nature, but a biological activity has not yet been identified for any member of the HIT family. Fragile histidine triad protein (FHIT) and protein kinase C interacting protein (PKCI) were used in a structure-based approach to elucidate characteristics of in vivo ligands and reactions. Crystallographic structures of apo, substrate analog, pentacovalent transition-state analog, and product states of both enzymes reveal a catalytic mechanism and define substrate characteristics required for catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both. The approach described here may be useful in identifying structure-function relations between protein families identified through genomics.

About this Structure

6FIT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207

Page seeded by OCA on Thu Mar 20 19:13:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6fit"
Personal tools