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7cat
From Proteopedia
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| - | [[Image:7cat.gif|left|200px]] | + | [[Image:7cat.gif|left|200px]] |
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| - | '''THE NADPH BINDING SITE ON BEEF LIVER CATALASE''' | + | {{Structure |
| + | |PDB= 7cat |SIZE=350|CAPTION= <scene name='initialview01'>7cat</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=NDP:NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NDP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE NADPH BINDING SITE ON BEEF LIVER CATALASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 7CAT is a [ | + | 7CAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CAT OCA]. |
==Reference== | ==Reference== | ||
| - | The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:[http:// | + | The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3856839 3856839] |
[[Category: Catalase]] | [[Category: Catalase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxidoreductase (h2o2 acceptor)]] | [[Category: oxidoreductase (h2o2 acceptor)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:14:20 2008'' |
Revision as of 17:14, 20 March 2008
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| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Catalase, with EC number 1.11.1.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE NADPH BINDING SITE ON BEEF LIVER CATALASE
Overview
Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery.
About this Structure
7CAT is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:3856839
Page seeded by OCA on Thu Mar 20 19:14:20 2008
