7pck
From Proteopedia
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| - | [[Image:7pck.gif|left|200px]] | + | [[Image:7pck.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF WILD TYPE HUMAN PROCATHEPSIN K''' | + | {{Structure |
| + | |PDB= 7pck |SIZE=350|CAPTION= <scene name='initialview01'>7pck</scene>, resolution 3.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Cathepsin_K Cathepsin K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.38 3.4.22.38] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF WILD TYPE HUMAN PROCATHEPSIN K''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 7PCK is a [ | + | 7PCK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PCK OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of wild-type human procathepsin K., Sivaraman J, Lalumiere M, Menard R, Cygler M, Protein Sci. 1999 Feb;8(2):283-90. PMID:[http:// | + | Crystal structure of wild-type human procathepsin K., Sivaraman J, Lalumiere M, Menard R, Cygler M, Protein Sci. 1999 Feb;8(2):283-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10048321 10048321] |
[[Category: Cathepsin K]] | [[Category: Cathepsin K]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: Menard, R.]] | [[Category: Menard, R.]] | ||
[[Category: Sivaraman, J.]] | [[Category: Sivaraman, J.]] | ||
| - | [[Category: cysteine | + | [[Category: cysteine protease]] |
[[Category: hydrolase (thiol protease)]] | [[Category: hydrolase (thiol protease)]] | ||
[[Category: procathepsin k]] | [[Category: procathepsin k]] | ||
[[Category: proregion]] | [[Category: proregion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:14:57 2008'' |
Revision as of 17:14, 20 March 2008
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| , resolution 3.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Cathepsin K, with EC number 3.4.22.38 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF WILD TYPE HUMAN PROCATHEPSIN K
Contents |
Overview
Cathepsin K is a lysosomal cysteine protease belonging to the papain superfamily. It has been implicated as a major mediator of osteoclastic bone resorption. Wild-type human procathepsin K has been crystallized in a glycosylated and a deglycosylated form. The latter crystals diffract better, to 3.2 A resolution, and contain four molecules in the asymmetric unit. The structure was solved by molecular replacement and refined to an R-factor of 0.194. The N-terminal fragment of the proregion forms a globular domain while the C-terminal segment is extended and shows substantial flexibility. The proregion interacts with the enzyme along the substrate binding groove and along the proregion binding loop (residues Ser138-Asn156). It binds to the active site in the opposite direction to that of natural substrates. The overall binding mode of the proregion to cathepsin K is similar to that observed in cathepsin L, caricain, and cathepsin B, but there are local differences that likely contribute to the specificity of these proregions for their cognate enzymes. The main observed difference is in the position of the short helix alpha3p (67p-75p), which occupies the S' subsites. As in the other proenzymes, the proregion utilizes the S2 subsite for anchoring by placing a leucine side chain there, according to the specificity of cathepsin K toward its substrate.
Disease
Known disease associated with this structure: Pycnodysostosis OMIM:[601105]
About this Structure
7PCK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of wild-type human procathepsin K., Sivaraman J, Lalumiere M, Menard R, Cygler M, Protein Sci. 1999 Feb;8(2):283-90. PMID:10048321
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