4wxh

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'''Unreleased structure'''
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==Carminomycin-4-O-methyltransferase (DnrK) variant (298Ser insert) in complex with S-adenosyl-L-homocystein (SAH) and aclacinomycin T==
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<StructureSection load='4wxh' size='340' side='right' caption='[[4wxh]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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The entry 4wxh is ON HOLD until Paper Publication
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4wxh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WXH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WXH FirstGlance]. <br>
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Authors: Metsa-Ketela, M., Niiranen, L.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3VL:METHYL+(1R,2R,4S)-2-ETHYL-2,5,7-TRIHYDROXY-6,11-DIOXO-4-{[2,3,6-TRIDEOXY-3-(DIMETHYLAMINO)-ALPHA-L-LYXO-HEXOPYRANOSYL]OXY}-1,2,3,4,6,11-HEXAHYDROTETRACENE-1-CARBOXYLATE'>3VL</scene>, <scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tw2|1tw2]], [[1tw3|1tw3]]</td></tr>
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Description: Carminomycin-4-O-methyltransferase (DnrK) variant (298Ser insert) in complex with S-adenosyl-L-homocystein (SAH) and aclacinomycin T
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carminomycin_4-O-methyltransferase Carminomycin 4-O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.292 2.1.1.292] </span></td></tr>
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[[Category: Unreleased Structures]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wxh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wxh RCSB], [http://www.ebi.ac.uk/pdbsum/4wxh PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/DNRK_STRPE DNRK_STRPE]] Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.<ref>PMID:15273252</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Carminomycin 4-O-methyltransferase]]
[[Category: Metsa-Ketela, M]]
[[Category: Metsa-Ketela, M]]
[[Category: Niiranen, L]]
[[Category: Niiranen, L]]
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[[Category: Antibiotic binding]]
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[[Category: Methyltransferase]]
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[[Category: Mono-oxygenase]]
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[[Category: Sah binding]]
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[[Category: Transferase]]

Revision as of 14:50, 29 July 2015

Carminomycin-4-O-methyltransferase (DnrK) variant (298Ser insert) in complex with S-adenosyl-L-homocystein (SAH) and aclacinomycin T

4wxh, resolution 1.90Å

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