8mht
From Proteopedia
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| - | [[Image:8mht.gif|left|200px]] | + | [[Image:8mht.gif|left|200px]] |
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| - | '''CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX''' | + | {{Structure |
| + | |PDB= 8mht |SIZE=350|CAPTION= <scene name='initialview01'>8mht</scene>, resolution 2.76Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 8MHT is a [ | + | 8MHT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8MHT OCA]. |
==Reference== | ==Reference== | ||
| - | Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base., O'Gara M, Horton JR, Roberts RJ, Cheng X, Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:[http:// | + | Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base., O'Gara M, Horton JR, Roberts RJ, Cheng X, Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9783745 9783745] |
[[Category: Deleted entry]] | [[Category: Deleted entry]] | ||
[[Category: Haemophilus haemolyticus]] | [[Category: Haemophilus haemolyticus]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:15:50 2008'' |
Revision as of 17:15, 20 March 2008
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| , resolution 2.76Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Deleted entry, with EC number 2.1.1.73 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX
Overview
Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.
About this Structure
8MHT is a Single protein structure of sequence from Haemophilus haemolyticus. Full crystallographic information is available from OCA.
Reference
Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base., O'Gara M, Horton JR, Roberts RJ, Cheng X, Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:9783745
Page seeded by OCA on Thu Mar 20 19:15:50 2008
