3jao

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'''Unreleased structure'''
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==Ciliary microtubule doublet==
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<StructureSection load='3jao' size='340' side='right' caption='[[3jao]], [[Resolution|resolution]] 23.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jao]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jao OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jao RCSB], [http://www.ebi.ac.uk/pdbsum/3jao PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microtubule doublet (MTD) is the main skeleton of cilia/flagella. Many proteins, such as dyneins and radial spokes, bind to MTD, and generate or regulate force. While the structure of the reconstituted microtubule has been solved at atomic resolution, nature of the axonemal MTD is still unclear. There are a few hypotheses of the lattice arrangement of its alpha- and beta-tubulins, but it has not been described how dyneins and radial spokes bind to MTD. In this study, we analyzed the three-dimensional structure of Tetrahymena MTD at approximately 19 A resolution by single particle cryo-electron microscopy. To identify alpha- and beta-tubulins, we combined image analysis of MTD with specific kinesin decoration. This work reveals that alpha- and beta-tubulins form a B-lattice arrangement in the entire MTD with a seam at the outer junction. We revealed the unique way in which inner arm dyneins, radial spokes, and proteins inside MTD bind and bridge protofilaments.
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The entry 3jao is ON HOLD until Paper Publication
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alpha- and beta-Tubulin Lattice of the Axonemal Microtubule Doublet and Binding Proteins Revealed by Single Particle Cryo-Electron Microscopy and Tomography.,Maheshwari A, Obbineni JM, Bui KH, Shibata K, Toyoshima YY, Ishikawa T Structure. 2015 Jul 23. pii: S0969-2126(15)00270-1. doi:, 10.1016/j.str.2015.06.017. PMID:26211611<ref>PMID:26211611</ref>
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Authors: Maheshwari, A., Obbineni, J.M., Bui, K.H., Shibata, K., Toyoshima, Y.Y., Ishikawa, T.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Ciliary microtubule doublet
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Tetrahymena thermophila]]
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[[Category: Bui, K H]]
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[[Category: Ishikawa, T]]
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[[Category: Maheshwari, A]]
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[[Category: Obbineni, J M]]
[[Category: Shibata, K]]
[[Category: Shibata, K]]
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[[Category: Obbineni, J.M]]
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[[Category: Toyoshima, Y Y]]
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[[Category: Toyoshima, Y.Y]]
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[[Category: Cilia]]
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[[Category: Maheshwari, A]]
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[[Category: Microtubule doublet]]
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[[Category: Bui, K.H]]
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[[Category: Structural protein]]
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[[Category: Ishikawa, T]]
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[[Category: Tubulin]]

Revision as of 20:02, 5 August 2015

Ciliary microtubule doublet

3jao, resolution 23.00Å

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