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4xoj

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'''Unreleased structure'''
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==Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)==
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<StructureSection load='4xoj' size='340' side='right' caption='[[4xoj]], [[Resolution|resolution]] 0.91&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4xoj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XOJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xoj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xoj RCSB], [http://www.ebi.ac.uk/pdbsum/4xoj PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/SFTI1_HELAN SFTI1_HELAN]] Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.<ref>PMID:10390350</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Serine proteinase catalyzed peptide splicing was demonstrated in both, one and two-peptide-chain (C- and N-terminal peptide chains linked by a disulfide bridge) trypsin inhibitor SFTI-1 analogues. In the second series, peptide splicing, with catalytic amount of proteinase, was observed only when formation of acyl-enzyme intermediate was preceded by the hydrolysis of the substrate Lys-Ser peptide bond. Here we presented that at an equimolar amount of the proteinase, splicing occurred in all two-peptide-chain analogues. This conclusion was supported by high resolution crystal structures of selected analogues in complex with trypsin. We showed that the process followed a direct transpeptidation mechanism. It means that the acyl-enzyme intermediate was formed and was immediately utilized for a new peptide bond formation, and products associated with the hydrolysis of acyl-enzyme were not observed. The demonstrated peptide splicing was a sequence- not structure-specific.
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The entry 4xoj is ON HOLD until Paper Publication
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Investigation of serine proteinase catalyzed peptide splicing in analogues of sunflower trypsin inhibitor 1 (SFTI-1).,Karna N, Malicki S, Debowski D, Golik P, Gitlin A, Grudnik P, Wladyka B, Brzozowski K, Dubin G, Rolka K, Legowska A Chembiochem. 2015 Jul 25. doi: 10.1002/cbic.201500296. PMID:26212347<ref>PMID:26212347</ref>
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Authors: Golik, P., Malicki, S., Grudnik, P., Karna, N., Debowski, D., Legowska, A., Wladyka, B., Gitlin, A., Brzozowski, K., Dubin, G., Rolka, K.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Grudnik, P]]
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__TOC__
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[[Category: Malicki, S]]
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Trypsin]]
[[Category: Brzozowski, K]]
[[Category: Brzozowski, K]]
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[[Category: Karna, N]]
 
[[Category: Debowski, D]]
[[Category: Debowski, D]]
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[[Category: Rolka, K]]
 
[[Category: Dubin, G]]
[[Category: Dubin, G]]
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[[Category: Gitlin, A]]
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[[Category: Golik, P]]
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[[Category: Grudnik, P]]
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[[Category: Karna, N]]
[[Category: Legowska, A]]
[[Category: Legowska, A]]
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[[Category: Malicki, S]]
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[[Category: Rolka, K]]
[[Category: Wladyka, B]]
[[Category: Wladyka, B]]
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[[Category: Golik, P]]
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[[Category: Hydrolase]]
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[[Category: Gitlin, A]]
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[[Category: Inhibitor]]
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[[Category: Protease]]
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[[Category: Sfti]]
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[[Category: Splicing]]

Revision as of 14:37, 12 August 2015

Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)

4xoj, resolution 0.91Å

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