4uwm

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'''Unreleased structure'''
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==Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.==
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<StructureSection load='4uwm' size='340' side='right' caption='[[4uwm]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4uwm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UWM FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uwm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uwm RCSB], [http://www.ebi.ac.uk/pdbsum/4uwm PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/C16MO_PSEPU C16MO_PSEPU]] Involved in the degradation of (-)-camphor. Catalyzes the lactonization of the 3,6-diketocamphane via the Baeyer-Villiger oxidation to produce the unstable lactone (-)-5-oxo-1,2-campholide that presumably undergoes spontaneous hydrolysis to form 2-oxo-delta(3)-4,5,5-trimethylcyclopentenylacetic acid. It acts only on bicyclic ketones.<ref>PMID:22286514</ref> <ref>PMID:8515237</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The oxygenating constituent of the 3,6-diketocamphane monooxygenase isozyme from Pseudomonas putida NCIMB 10007 has been crystallized under two different conditions. Crystals were initially grown from polyethylene glycol (PEG) 8000 and sodium acetate using the vapour-phase diffusion method. The crystals were of orthorhombic P212121 space group, with cell dimensions a = 55.8, b = 94.5 and c = 163.7 A and diffracted to 2.8 A resolution. More recently, improved crystals, which diffracted beyond 2 A, have been grown from ammonium sulfate. These crystals also belong to the orthorhombic P212121 space group, with cell dimensions of a = 54.6, b = 93.2 and c = 154. 1 A. A full native data set to 2.5 A resolution has been collected from the ammonium sulfate grown crystals.
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The entry 4uwm is ON HOLD until Paper Publication
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Crystallization and preliminary X-ray diffraction studies of the oxygenating subunit of 3,6-diketocamphane monooxygenase from Pseudomonas putida.,McGhie EJ, Isupov MN, Schroder E, Littlechild JA Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):1035-8. PMID:9757131<ref>PMID:9757131</ref>
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Authors: Isupov, M.N., Schroeder, E., Gibson, R.P., Beecher, J., Donadio, G., Saneei, V., Dcunha, S., McGhie, E.J., Sayer, C., Davenport, C.F., Lau, P.C., Hasegawa, Y., Iwaki, H., Kadow, M., Loschinski, K., Bornscheuer, U.T., Bourenkov, G., Littlechild, J.A.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Bornscheuer, U.T]]
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__TOC__
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</StructureSection>
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[[Category: Beecher, J]]
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[[Category: Bornscheuer, U T]]
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[[Category: Bourenkov, G]]
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[[Category: Davenport, C F]]
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[[Category: Dcunha, S]]
[[Category: Donadio, G]]
[[Category: Donadio, G]]
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[[Category: Davenport, C.F]]
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[[Category: Gibson, R P]]
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[[Category: Dcunha, S]]
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[[Category: Isupov, M.N]]
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[[Category: Lau, P.C]]
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[[Category: Mcghie, E.J]]
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[[Category: Kadow, M]]
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[[Category: Hasegawa, Y]]
[[Category: Hasegawa, Y]]
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[[Category: Beecher, J]]
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[[Category: Isupov, M N]]
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[[Category: Bourenkov, G]]
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[[Category: Gibson, R.P]]
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[[Category: Iwaki, H]]
[[Category: Iwaki, H]]
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[[Category: Kadow, M]]
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[[Category: Lau, P C]]
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[[Category: Littlechild, J A]]
[[Category: Loschinski, K]]
[[Category: Loschinski, K]]
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[[Category: McGhie, E J]]
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[[Category: Saneei, V]]
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[[Category: Sayer, C]]
[[Category: Schroeder, E]]
[[Category: Schroeder, E]]
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[[Category: Littlechild, J.A]]
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[[Category: Biocatalysis]]
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[[Category: Sayer, C]]
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[[Category: Oxidoreductase]]
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[[Category: Saneei, V]]
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Revision as of 13:28, 26 August 2015

Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.

4uwm, resolution 1.90Å

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