4r9i
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal structure of cysteine proteinase inhibitor Serpin18 from Bombyx mori== |
| + | <StructureSection load='4r9i' size='340' side='right' caption='[[4r9i]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4r9i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R9I FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r9i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r9i RCSB], [http://www.ebi.ac.uk/pdbsum/4r9i PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Serpins generally serve as inhibitors that utilize a mobile reactive center loop (RCL) as bait to trap protease targets. Here, we present the crystal structure of serpin18 from Bombyx mori at 1.65 A resolution, which has a very short and stable RCL. Activity analysis showed that the inhibitory target of serpin18 is a cysteine protease rather than a serine protease. Notably, this inhibitiory reaction results from the formation of an intermediate complex, which then follows for the digestion of protease and inhibitor into small fragments. This activity differs from previously reported modes of inhibition for serpins. Our findings have thus provided novel structural insights into the unique inhibitory mechanism of serpin18. Furthermore, one physiological target of serpin18, fibroinase, was identified, which enables us to better define the potential role for serpin18 in regulating fibroinase activity during B. mori development. | ||
| - | + | Structural insights into the unique inhibitory mechanism of the silkworm protease inhibitor serpin18.,Guo PC, Dong Z, Zhao P, Zhang Y, He H, Tan X, Zhang W, Xia Q Sci Rep. 2015 Jul 7;5:11863. doi: 10.1038/srep11863. PMID:26148664<ref>PMID:26148664</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Guo, P C]] | ||
| + | [[Category: He, H W]] | ||
| + | [[Category: Xia, Q Y]] | ||
[[Category: Zhao, P]] | [[Category: Zhao, P]] | ||
| - | [[Category: | + | [[Category: Hydrolase inhibitor]] |
| - | [[Category: | + | [[Category: Proteinase inhibitor]] |
| - | + | ||
Revision as of 11:14, 9 September 2015
Crystal structure of cysteine proteinase inhibitor Serpin18 from Bombyx mori
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