1n38
From Proteopedia
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|PDB= 1n38 |SIZE=350|CAPTION= <scene name='initialview01'>1n38</scene>, resolution 2.8Å | |PDB= 1n38 |SIZE=350|CAPTION= <scene name='initialview01'>1n38</scene>, resolution 2.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U3H:3 | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U3H:3'-DEOXY-URIDINE+5'-TRIPHOSPHATE'>U3H</scene> and <scene name='pdbligand=CH1:3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE'>CH1</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= L1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10886 Mammalian orthoreovirus 3]) | |GENE= L1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10886 Mammalian orthoreovirus 3]) | ||
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[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:51:01 2008'' |
Revision as of 10:51, 23 March 2008
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| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | L1 (Mammalian orthoreovirus 3) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed
Overview
The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.
About this Structure
1N38 is a Single protein structure of sequence from Mammalian orthoreovirus 3. Full crystallographic information is available from OCA.
Reference
RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:12464184
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