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2l0j

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Revision as of 00:20, 10 September 2015

Solid State NMR structure of the M2 proton channel from Influenza A Virus in hydrated lipid bilayer

Structural highlights

2l0j is a 4 chain structure with sequence from I72a2. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	M (I72A2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[M2_I72A2] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).^[1]

Publication Abstract from PubMed

The M2 protein from the influenza A virus, an acid-activated proton-selective channel, has been the subject of numerous conductance, structural, and computational studies. However, little is known at the atomic level about the heart of the functional mechanism for this tetrameric protein, a His(37)-Trp(41) cluster. We report the structure of the M2 conductance domain (residues 22 to 62) in a lipid bilayer, which displays the defining features of the native protein that have not been attainable from structures solubilized by detergents. We propose that the tetrameric His(37)-Trp(41) cluster guides protons through the channel by forming and breaking hydrogen bonds between adjacent pairs of histidines and through specific interactions of the histidines with the tryptophan gate. This mechanism explains the main observations on M2 proton conductance.

Insight into the mechanism of the influenza A proton channel from a structure in a lipid bilayer.,Sharma M, Yi M, Dong H, Qin H, Peterson E, Busath DD, Zhou HX, Cross TA Science. 2010 Oct 22;330(6003):509-12. PMID:20966252^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Holsinger LJ, Nichani D, Pinto LH, Lamb RA. Influenza A virus M2 ion channel protein: a structure-function analysis. J Virol. 1994 Mar;68(3):1551-63. PMID:7508997
↑ Sharma M, Yi M, Dong H, Qin H, Peterson E, Busath DD, Zhou HX, Cross TA. Insight into the mechanism of the influenza A proton channel from a structure in a lipid bilayer. Science. 2010 Oct 22;330(6003):509-12. PMID:20966252 doi:10.1126/science.1191750

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l0j"

@@ Line 2: / Line 2: @@
 <StructureSection load='2l0j' size='340' side='right' caption='[[2l0j]], [[NMR_Ensembles_of_Models | 8 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2l0j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2l0j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/I72a2 I72a2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0J FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381517 I72A2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l0j RCSB], [http://www.ebi.ac.uk/pdbsum/2l0j PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0j OCA], [http://pdbe.org/2l0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l0j RCSB], [http://www.ebi.ac.uk/pdbsum/2l0j PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2l0j" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Influenza a virus]]
+[[Category: I72a2]]
 [[Category: Busath, D D]]
 [[Category: Cross, T A]]

2l0j

From Proteopedia

Revision as of 00:20, 10 September 2015

Solid State NMR structure of the M2 proton channel from Influenza A Virus in hydrated lipid bilayer

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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