1pfi

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|PDB= 1pfi |SIZE=350|CAPTION= <scene name='initialview01'>1pfi</scene>, resolution 3.0&Aring;
|PDB= 1pfi |SIZE=350|CAPTION= <scene name='initialview01'>1pfi</scene>, resolution 3.0&Aring;
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|LIGAND= <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>
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|LIGAND= <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>
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[[Category: helical virus]]
[[Category: helical virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:23:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:12:07 2008''

Revision as of 11:12, 23 March 2008

Image:1pfi.gif


PDB ID 1pfi

Drag the structure with the mouse to rotate
, resolution 3.0Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES


Overview

The helical path of the DNA in filamentous bacteriophage Pf1 was deduced from different kinds of existing structural information, including results from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as the surrounding helix of protein subunits; the rise and rotation per nucleotides are 6.1 angstroms and 132 degrees, respectively; and the phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA structure and extensive additional information about the protein, a model of the virion is proposed. In the model, the DNA bases reach out, into the protein, and the lysine and arginine side chains reach in, between the DNA bases, to stabilize the paraxial phosphate charges; the conformation of the protein subunit is a combination of alpha and 3(10) helices.

About this Structure

1PFI is a Single protein structure of sequence from Pseudomonas phage pf1. Full crystallographic information is available from OCA.

Reference

Pf1 virus structure: helical coat protein and DNA with paraxial phosphates., Liu DJ, Day LA, Science. 1994 Jul 29;265(5172):671-4. PMID:8036516

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