1uon

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|PDB= 1uon |SIZE=350|CAPTION= <scene name='initialview01'>1uon</scene>
|PDB= 1uon |SIZE=350|CAPTION= <scene name='initialview01'>1uon</scene>
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CH1:3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE'>CH1</scene>
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CH1:3&#39;-DEOXY-CYTIDINE-5&#39;-TRIPHOSPHATE'>CH1</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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[[Category: reovirus]]
[[Category: reovirus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:34:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:53:39 2008''

Revision as of 11:53, 23 March 2008

Image:1uon.gif


PDB ID 1uon

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Ligands: and
Coordinates: save as pdb, mmCIF, xml



REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION


Overview

Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral polymerases packaged within the viral core to transcribe its ten distinct plus-strand RNAs. To localize these polymerases, the structure of the reovirion was refined to a resolution of 7.6 A by cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction. X-ray crystal models of reovirus proteins, including polymerase lambda 3, were then fitted into the density map. Each copy of lambda 3 was found anchored to the inner surface of the icosahedral core shell, making major contacts with three molecules of shell protein lambda 1 and overlapping, but not centering on, a five-fold axis. The overlap explains why only one copy of lambda 3 is bound per vertex. lambda 3 is furthermore oriented with its transcript exit channel facing a small channel through the lambda 1 shell, suggesting how the nascent RNA is passed into the large external cavity of the pentameric capping enzyme complex formed by protein lambda 2.

About this Structure

1UON is a Protein complex structure of sequences from Reovirus sp.. Full crystallographic information is available from OCA.

Reference

Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 A., Zhang X, Walker SB, Chipman PR, Nibert ML, Baker TS, Nat Struct Biol. 2003 Dec;10(12):1011-8. Epub 2003 Nov 9. PMID:14608373

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