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1wdy

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|PDB= 1wdy |SIZE=350|CAPTION= <scene name='initialview01'>1wdy</scene>, resolution 1.80&Aring;
|PDB= 1wdy |SIZE=350|CAPTION= <scene name='initialview01'>1wdy</scene>, resolution 1.80&Aring;
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|LIGAND= <scene name='pdbligand=25A:5'-O-MONOPHOSPHORYLADENYLYL(2'->5')ADENYLYL(2'->5')ADENOSINE'>25A</scene>
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|LIGAND= <scene name='pdbligand=25A:5&#39;-O-MONOPHOSPHORYLADENYLYL(2&#39;-&#62;5&#39;)ADENYLYL(2&#39;-&#62;5&#39;)ADENOSINE'>25A</scene>
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[[Category: rna-binding]]
[[Category: rna-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:55:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:06:29 2008''

Revision as of 12:06, 23 March 2008

Image:1wdy.jpg


PDB ID 1wdy

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Crystal structure of ribonuclease


Contents

Overview

An interferon-induced endoribonuclease, ribonuclease L (RNase L), is implicated in both the molecular mechanism of action of interferon and the fundamental control of RNA stability in mammalian cells. RNase L is catalytically active only after binding to an unusual activator molecule containing a 5'-phosphorylated 2',5'-linked oligoadenylate (2-5A), in the N-terminal half. Here, we report the crystal structure of the N-terminal ankyrin repeat domain (ANK) of human RNase L complexed with the activator 2-5A. This is the first structural view of an ankyrin repeat structure directly interacting with a nucleic acid, rather than with a protein. The ANK domain folds into eight ankyrin repeat elements and forms an extended curved structure with a concave surface. The 2-5A molecule is accommodated at a concave site and directly interacts with ankyrin repeats 2-4. Interestingly, two structurally equivalent 2-5A binding motifs are found at repeats 2 and 4. The structural basis for 2-5A recognition by ANK is essential for designing stable 2-5As with a high likelihood of activating RNase L.

Disease

Known diseases associated with this structure: Prostate cancer 1, 176807 OMIM:[180435]

About this Structure

1WDY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for recognition of 2',5'-linked oligoadenylates by human ribonuclease L., Tanaka N, Nakanishi M, Kusakabe Y, Goto Y, Kitade Y, Nakamura KT, EMBO J. 2004 Oct 13;23(20):3929-38. Epub 2004 Sep 23. PMID:15385955

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