2bt6

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|PDB= 2bt6 |SIZE=350|CAPTION= <scene name='initialview01'>2bt6</scene>, resolution 1.50&Aring;
|PDB= 2bt6 |SIZE=350|CAPTION= <scene name='initialview01'>2bt6</scene>, resolution 1.50&Aring;
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+B'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene> and <scene name='pdbligand=RUA:(4'-METHYL-2,2'BIPYRIDINE)BIS(2,2'-BIPYRIDINE)'>RUA</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene> and <scene name='pdbligand=RUA:(4&#39;-METHYL-2,2&#39;BIPYRIDINE)BIS(2,2&#39;-BIPYRIDINE)'>RUA</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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[[Category: transit peptide]]
[[Category: transit peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:06:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:47:43 2008''

Revision as of 12:47, 23 March 2008

Image:2bt6.gif


PDB ID 2bt6

Drag the structure with the mouse to rotate
, resolution 1.50Å
Sites:
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN


Overview

Bovine adrenodoxin (Adx) plays an important role in the electron-transfer process in the mitochondrial steroid hydroxylase system of the bovine adrenal cortex. Using electron paramagnetic resonance (EPR) spectroscopy, we showed that photoreduction of the [2Fe-2S] cluster of Adx via (4'-methyl-2,2'-bipyridine)bis(2,2'-bipyridine)ruthenium(II) [Ru(bpy)2(mbpy)] covalently attached to the protein surface can be used as a new approach to probe the "shuttle" hypothesis for the electron transfer by Adx. The 1.5 A resolution crystal structure of a 1:1 Ru(bpy)2(mbpy)-Adx(1-108) complex reveals the site of modification, Cys95, and allows to predict the possible intramolecular electron-transfer pathways within the complex. Photoreduction of uncoupled Adx, mutant Adx(1-108), and Ru(bpy)2(mbpy)-Adx(1-108) using safranin T as the mediating electron donor suggests that two electrons are transferred from the dye to Adx. The intramolecular photoreduction rate constant for the ruthenated Adx has been determined and is discussed according to the predicted pathways.

About this Structure

2BT6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure., Halavaty A, Muller JJ, Contzen J, Jung C, Hannemann F, Bernhardt R, Galander M, Lendzian F, Heinemann U, Biochemistry. 2006 Jan 24;45(3):709-18. PMID:16411746

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