1eyo

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(Difference between revisions)

Revision as of 13:41, 10 September 2015

SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA

Structural highlights

1eyo is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[C7A_CONTU] By structural similarity with conotoxin GS, may inhibit the sodium channel (Nav). No effect was observed upon intracranial injections into mice and intraperitoneal injections into goldfish (25 ug).

Publication Abstract from PubMed

The three-dimensional solution structure of conotoxin TVIIA, a 30-residue polypeptide from the venom of the piscivorous cone snail Conus tulipa, has been determined using 2D 1H NMR spectroscopy. TVIIA contains six cysteine residues which form a 'four-loop' structural framework common to many peptides from Conus venoms including the omega-, delta-, kappa-, and muO-conotoxins. However, TVIIA does not belong to these well-characterized pharmacological classes of conotoxins, but displays high sequence identity with conotoxin GS, a muscle sodium channel blocker from Conus geographus. Structure calculations were based on 562 interproton distance restraints inferred from NOE data, together with 18 backbone and nine side-chain torsion angle restraints derived from spin-spin coupling constants. The final family of 20 structures had mean pairwise rms differences over residues 2-27 of 0.18+/-0.05 A for the backbone atoms and 1.39+/-0.33 A for all heavy atoms. The structure consists of a triple-stranded, antiparallel beta sheet with +2x, -1 topology (residues 7-9, 16-20 and 23-27) and several beta turns. The core of the molecule is formed by three disulfide bonds which form a cystine knot motif common to many toxic and inhibitory polypeptides. The global fold, molecular shape and distribution of amino-acid sidechains in TVIIA is similar to that previously reported for conotoxin GS, and comparison with other four-loop conotoxin structures provides further indication that TVIIA and GS represent a new and distinct subgroup of this structural family. The structure of TVIIA determined in this study provides the basis for determining a structure-activity relationship for these molecules and their interaction with target receptors.

Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure.,Hill JM, Alewood PF, Craik DJ Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:10903497^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hill JM, Alewood PF, Craik DJ. Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure. Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:10903497

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1eyo"

Categories: Alewood, P F | Craik, D J | Hill, J M | Cystine knot motif | Toxin

@@ Line 4: / Line 4: @@
 <table><tr><td colspan='2'>[[1eyo]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EYO FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eyo RCSB], [http://www.ebi.ac.uk/pdbsum/1eyo PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyo OCA], [http://pdbe.org/1eyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eyo RCSB], [http://www.ebi.ac.uk/pdbsum/1eyo PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CX7A_CONTU CX7A_CONTU]] By structural similarity with conotoxin GS, may inhibit the sodium channel (Nav). No effect was observed upon intracranial injections into mice and intraperitoneal injections into goldfish (25 ug).
+[[http://www.uniprot.org/uniprot/C7A_CONTU C7A_CONTU]] By structural similarity with conotoxin GS, may inhibit the sodium channel (Nav). No effect was observed upon intracranial injections into mice and intraperitoneal injections into goldfish (25 ug).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1eyo" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

1eyo

From Proteopedia

Revision as of 13:41, 10 September 2015

SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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