1uvk
From Proteopedia
(Difference between revisions)
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| - | + | ==The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase phi6p2 dead-end complex== | |
| - | + | <StructureSection load='1uvk' size='340' side='right' caption='[[1uvk]], [[Resolution|resolution]] 2.45Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1uvk]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UVK FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uvk OCA], [http://pdbe.org/1uvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uvk RCSB], [http://www.ebi.ac.uk/pdbsum/1uvk PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+. | ||
| - | + | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.,Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM Structure. 2004 Feb;12(2):307-16. PMID:14962391<ref>PMID:14962391</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| + | <div class="pdbe-citations 1uvk" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[RNA polymerase|RNA polymerase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bpph6]] | [[Category: Bpph6]] | ||
[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
| - | [[Category: Bamford, D | + | [[Category: Bamford, D]] |
| - | [[Category: Butcher, S | + | [[Category: Butcher, S]] |
| - | [[Category: Grimes, J M | + | [[Category: Grimes, J M]] |
| - | [[Category: Makeyev, E V | + | [[Category: Makeyev, E V]] |
| - | [[Category: Salgado, P S | + | [[Category: Salgado, P S]] |
| - | [[Category: Stuart, D I | + | [[Category: Stuart, D I]] |
[[Category: Oligonucleotide]] | [[Category: Oligonucleotide]] | ||
[[Category: Polymerase]] | [[Category: Polymerase]] | ||
[[Category: Polymerase-complex]] | [[Category: Polymerase-complex]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 14:20, 10 September 2015
The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase phi6p2 dead-end complex
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