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1xfn

From Proteopedia

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Revision as of 15:19, 10 September 2015

NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part

Structural highlights

1xfn is a 1 chain structure with sequence from Dsm 244. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1odv, 2phy, 1xfq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated.

The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein.,Bernard C, Houben K, Derix NM, Marks D, van der Horst MA, Hellingwerf KJ, Boelens R, Kaptein R, van Nuland NA Structure. 2005 Jul;13(7):953-62. PMID:16004868^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bernard C, Houben K, Derix NM, Marks D, van der Horst MA, Hellingwerf KJ, Boelens R, Kaptein R, van Nuland NA. The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein. Structure. 2005 Jul;13(7):953-62. PMID:16004868 doi:10.1016/j.str.2005.04.017

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xfn"

@@ Line 2: / Line 2: @@
 <StructureSection load='1xfn' size='340' side='right' caption='[[1xfn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1xfn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XFN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1xfn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_244 Dsm 244]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XFN FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1odv|1odv]], [[2phy|2phy]], [[1xfq|1xfq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xfn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xfn RCSB], [http://www.ebi.ac.uk/pdbsum/1xfn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xfn OCA], [http://pdbe.org/1xfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xfn RCSB], [http://www.ebi.ac.uk/pdbsum/1xfn PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA]] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 25: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1xfn" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Halorhodospira halophila]]
+[[Category: Dsm 244]]
 [[Category: Bernard, C]]
 [[Category: Boelens, R]]

1xfn

From Proteopedia

Revision as of 15:19, 10 September 2015

NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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