1jf0

From Proteopedia

(Difference between revisions)

Revision as of 18:57, 10 September 2015

The Crystal Structure of Obelin from Obelia geniculata at 1.82 A Resolution

Structural highlights

1jf0 is a 1 chain structure with sequence from Black sea hydrozoan. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1el4, 1ej3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[OBL_OBELO] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia longissima has been determined and refined to 1.7 A resolution. Contrary to the prediction of a peroxide, the noncovalently bound substrate, coelenterazine, has only a single oxygen atom bound at the C2-position. The protein-coelenterazine 2-oxy complex observed in the crystals is photo-active because, in the presence of calcium ion, bioluminescence emission within the crystal is observed. This structure represents only the second de novo protein structure determined using the anomalous scattering signal of the sulfur substructure in the crystal. The method used here is theoretically different from that used for crambin in 1981 (4.72 kDa) and represents a significant advancement in protein crystal structure determination.

Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure.,Liu ZJ, Vysotski ES, Chen CJ, Rose JP, Lee J, Wang BC Protein Sci. 2000 Nov;9(11):2085-93. PMID:11152120^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu ZJ, Vysotski ES, Chen CJ, Rose JP, Lee J, Wang BC. Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure. Protein Sci. 2000 Nov;9(11):2085-93. PMID:11152120

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jf0"

@@ Line 5: / Line 5: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZH:C2-HYDROPEROXY-COELENTERAZINE'>CZH</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1el4|1el4]], [[1ej3|1ej3]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jf0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jf0 RCSB], [http://www.ebi.ac.uk/pdbsum/1jf0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jf0 OCA], [http://pdbe.org/1jf0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jf0 RCSB], [http://www.ebi.ac.uk/pdbsum/1jf0 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1jf0" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

1jf0

From Proteopedia

Revision as of 18:57, 10 September 2015

The Crystal Structure of Obelin from Obelia geniculata at 1.82 A Resolution

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox