1uqr
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The structure of the type II dehydroquinate dehydratase (DHQase) from, Actinobacillus pleuropneumoniae, the third enzyme of the shikimate, pathway, has been determined. Crystals diffracting to 1.7 A were obtained, in space and on earth using the counter-diffusion technique. The structure, was solved using molecular replacement and refined to high resolution. The, overall structure of the dodecameric enzyme is described and compared with, structures of DHQases from other bacteria. DHQases contain a flexible loop, that presumably closes over the active site upon substrate binding. The, enzyme can exist in an open or closed conformation. The present structure, displays the open conformation, with a sulfate anion bound in the active, site. The availability of this structure opens a route to . | + | The structure of the type II dehydroquinate dehydratase (DHQase) from, Actinobacillus pleuropneumoniae, the third enzyme of the shikimate, pathway, has been determined. Crystals diffracting to 1.7 A were obtained, in space and on earth using the counter-diffusion technique. The structure, was solved using molecular replacement and refined to high resolution. The, overall structure of the dodecameric enzyme is described and compared with, structures of DHQases from other bacteria. DHQases contain a flexible loop, that presumably closes over the active site upon substrate binding. The, enzyme can exist in an open or closed conformation. The present structure, displays the open conformation, with a sulfate anion bound in the active, site. The availability of this structure opens a route to structure-based, antibiotics targetting this pathogenic bacterium. |
==About this Structure== | ==About this Structure== | ||
| - | 1UQR is a | + | 1UQR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae] with SO4 and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UQR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: shikimate pathway]] | [[Category: shikimate pathway]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:43:50 2007'' |
Revision as of 12:38, 5 November 2007
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TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE
Overview
The structure of the type II dehydroquinate dehydratase (DHQase) from, Actinobacillus pleuropneumoniae, the third enzyme of the shikimate, pathway, has been determined. Crystals diffracting to 1.7 A were obtained, in space and on earth using the counter-diffusion technique. The structure, was solved using molecular replacement and refined to high resolution. The, overall structure of the dodecameric enzyme is described and compared with, structures of DHQases from other bacteria. DHQases contain a flexible loop, that presumably closes over the active site upon substrate binding. The, enzyme can exist in an open or closed conformation. The present structure, displays the open conformation, with a sulfate anion bound in the active, site. The availability of this structure opens a route to structure-based, antibiotics targetting this pathogenic bacterium.
About this Structure
1UQR is a Single protein structure of sequence from Actinobacillus pleuropneumoniae with SO4 and TRS as ligands. Active as 3-dehydroquinate dehydratase, with EC number 4.2.1.10 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae., Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:14993670
Page seeded by OCA on Mon Nov 5 14:43:50 2007
Categories: 3-dehydroquinate dehydratase | Actinobacillus pleuropneumoniae | Single protein | Afonina, E. | Bondt, H.De. | Garcia-Ruiz, J.M. | Gonzalez-Ramirez, L.A. | Gulnik, S. | Lopez-Jaramillo, J. | Maes, D. | Yu, B. | Zegers, I. | SO4 | TRS | Aromatic amino acid biosynthesis | Lyase | Shikimate pathway
