2csa

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Revision as of 20:40, 10 September 2015

Structure of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal

Structural highlights

2csa is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Disease

[ACM3_HUMAN] Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS) [MIM:100100]. EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.^[1]

Function

[ACM3_HUMAN] The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Publication Abstract from PubMed

Muscarinic acetylcholine receptors comprise a family of G-protein-coupled receptors that display differential localization in polarized epithelial cells. We identify a seven-residue sequence, Ala(275)-Val(281), in the third intracellular loop of the M(3) muscarinic receptor that mediates dominant, position-independent basolateral targeting in Madin-Darby canine kidney cells. Mutational analyses identify Glu(276), Phe(280), and Val(281) as critical residues within this sorting motif. Phe(280) and Val(281) comprise a novel dihydrophobic sorting signal as mutations of either residue singly or together with leucine do not disrupt basolateral targeting. Conversely, Glu(276) is required and cannot be substituted with alanine or aspartic acid. A 19-amino acid peptide representing the M(3) sorting signal and surrounding sequence was analyzed via two-dimensional nuclear magnetic resonance spectroscopy. Solution structures show that Glu(276) resides in a type IV beta-turn and the dihydrophobic sequence Phe(280)Val(281) adopts either a type I or IV beta-turn.

Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal.,Iverson HA, Fox D 3rd, Nadler LS, Klevit RE, Nathanson NM J Biol Chem. 2005 Jul 1;280(26):24568-75. Epub 2005 May 2. PMID:15870063^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Weber S, Thiele H, Mir S, Toliat MR, Sozeri B, Reutter H, Draaken M, Ludwig M, Altmuller J, Frommolt P, Stuart HM, Ranjzad P, Hanley NA, Jennings R, Newman WG, Wilcox DT, Thiel U, Schlingmann KP, Beetz R, Hoyer PF, Konrad M, Schaefer F, Nurnberg P, Woolf AS. Muscarinic Acetylcholine Receptor M3 Mutation Causes Urinary Bladder Disease and a Prune-Belly-like Syndrome. Am J Hum Genet. 2011 Nov 11;89(5):668-74. doi: 10.1016/j.ajhg.2011.10.007. PMID:22077972 doi:10.1016/j.ajhg.2011.10.007
↑ Iverson HA, Fox D 3rd, Nadler LS, Klevit RE, Nathanson NM. Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal. J Biol Chem. 2005 Jul 1;280(26):24568-75. Epub 2005 May 2. PMID:15870063 doi:http://dx.doi.org/M501264200

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2csa"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2csa]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CSA FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2csa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2csa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2csa RCSB], [http://www.ebi.ac.uk/pdbsum/2csa PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2csa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2csa OCA], [http://pdbe.org/2csa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2csa RCSB], [http://www.ebi.ac.uk/pdbsum/2csa PDBsum]</span></td></tr>
 </table>
 == Disease ==
@@ Line 17: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2csa" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

2csa

From Proteopedia

Revision as of 20:40, 10 September 2015

Structure of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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