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1bh4

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Revision as of 20:46, 10 September 2015

CIRCULIN A FROM CHASSALIA PARVIFLORA, NMR, 12 STRUCTURES

Structural highlights

1bh4 is a 1 chain structure with sequence from Chassalia parviflora. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[CIRA_CHAPA] Probably participates in a plant defense mechanism. Has antibiotic activity. Inhibits the cytopathic effects and replication of the human immunodeficiency virus. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The three-dimensional solution structure of circulin A, a 30 residue polypeptide from the African plant Chassalia parvifolia, has been determined using two-dimensional 1H-NMR spectroscopy. Circulin A was originally identified based upon its inhibition of the cytopathic effects and replication of the human immunodeficiency virus. Structural restraints consisting of 369 interproton distances inferred from nuclear Overhauser effects, and 21 backbone dihedral and nine chi1 angle restraints from spin-spin coupling constants were used as input for simulated annealing calculations and energy minimisation in the program X-PLOR. The final set of 12 structures had mean pairwise rms differences over the whole molecule of 0.91 A for the backbone atom, and 1.68 A for all heavy atoms. For the well-defined region encompassing residues 2-12 and 18-27, the corresponding values were 0.71 and 1.66 A, respectively. Circulin A adopts a compact structure consisting of beta-turns and a distorted segment of triple-stranded beta-sheet. Fluorescence spectroscopy provided additional evidence for a solvent-exposed Trp residue. The molecule is stabilised by three disulfide bonds, two of which form an embedded loop completed by the backbone fragments connecting the cysteine residues. A third disulfide bond threads through the centre of this loop to form a "cystine-knot" motif. This motif is present in a range of other biologically active proteins, including omega-contoxin GVIA and Cucurbita maxima trypsin inhibitor. Circulin A belongs to a novel class of macrocyclic peptides which have been isolated from plants in the Rubiaceae family. The global fold of circulin A is similar to kalata B1, the only member of this class for which a structure has previously been determined.

Solution structure by NMR of circulin A: a macrocyclic knotted peptide having anti-HIV activity.,Daly NL, Koltay A, Gustafson KR, Boyd MR, Casas-Finet JR, Craik DJ J Mol Biol. 1999 Jan 8;285(1):333-45. PMID:9878410^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Daly NL, Koltay A, Gustafson KR, Boyd MR, Casas-Finet JR, Craik DJ. Solution structure by NMR of circulin A: a macrocyclic knotted peptide having anti-HIV activity. J Mol Biol. 1999 Jan 8;285(1):333-45. PMID:9878410 doi:10.1006/jmbi.1998.2276

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bh4"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1bh4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chassalia_parviflora Chassalia parviflora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BH4 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bh4 RCSB], [http://www.ebi.ac.uk/pdbsum/1bh4 PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bh4 OCA], [http://pdbe.org/1bh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bh4 RCSB], [http://www.ebi.ac.uk/pdbsum/1bh4 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 25: / Line 25: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1bh4" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

1bh4

From Proteopedia

Revision as of 20:46, 10 September 2015

CIRCULIN A FROM CHASSALIA PARVIFLORA, NMR, 12 STRUCTURES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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