2p73
From Proteopedia
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|PDB= 2p73 |SIZE=350|CAPTION= <scene name='initialview01'>2p73</scene>, resolution 2.300Å | |PDB= 2p73 |SIZE=350|CAPTION= <scene name='initialview01'>2p73</scene>, resolution 2.300Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=UDP:URIDINE-5 | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= A64R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1]) | |GENE= A64R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1]) | ||
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[[Category: pbcv-1 a64r]] | [[Category: pbcv-1 a64r]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:37:19 2008'' |
Revision as of 13:37, 23 March 2008
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| , resolution 2.300Å | |||||||
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| Ligands: | and | ||||||
| Gene: | A64R (Paramecium bursaria Chlorella virus 1) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1
Overview
Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
About this Structure
2P73 is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:17850743
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