1gvg
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1GVG is a | + | 1GVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with FE, SO4, NO, AKG and PCX as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trifunctional enzyme]] | [[Category: trifunctional enzyme]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:44:50 2007'' |
Revision as of 12:39, 5 November 2007
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CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE
Overview
Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of, CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were, exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to, exposure with NO, the active site Fe(II) is octahedrally coordinated by a, water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the, side-chains of an aspartyl and two histidinyl residues. NO binds to the, position previously occupied by the 2OG 1-carboxylate concomitant with, rearrangement of the latter to the position previously occupied by the, displaced water.
About this Structure
1GVG is a Single protein structure of sequence from Streptomyces clavuligerus with FE, SO4, NO, AKG and PCX as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements., Zhang Z, Ren J, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ, FEBS Lett. 2002 Apr 24;517(1-3):7-12. PMID:12062399
Page seeded by OCA on Mon Nov 5 14:44:50 2007
Categories: Single protein | Streptomyces clavuligerus | Clifton, I.J. | Harlos, K. | Mckinnon, C.H. | Ren, J. | Schofield, C.J. | Zhang, Z.H. | AKG | FE | NO | PCX | SO4 | Clavaminate synthase 1 | Jelly roll | Lyase | Nitric oxide | Oxidoreductase | Oxygenase | Trifunctional enzyme
