1xdo

From Proteopedia

(Difference between revisions)

Revision as of 01:16, 11 September 2015

Crystal Structure of Escherichia coli Polyphosphate Kinase

Structural highlights

1xdo is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1xdp
Gene:	ppk ("Bacillus coli" Migula 1895)
Activity:	Polyphosphate kinase, with EC number 2.7.4.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[PPK_ECOLI] Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can also act in the reverse direction to form ATP in the presence of excess ADP. Can also use GTP instead of ATP; but the efficiency of GTP is 5% that of ATP.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Polyphosphate (polyP), a linear polymer of hundreds of orthophosphate residues, exists in all tested cells in nature, from pathogenic bacteria to mammals. In bacteria, polyP has a crucial role in stress responses and stationary-phase survival. Polyphosphate kinase (PPK) is the principal enzyme that catalyses the synthesis of polyP in bacteria. It has been shown that PPK is required for bacterial motility, biofilm formation and the production of virulence factors. PPK inhibitors may thus provide a unique therapeutic opportunity against antibiotic-resistant pathogens. Here, we report crystal structures of full-length Escherichia coli PPK and its complex with AMPPNP (beta-gamma-imidoadenosine 5-phosphate). PPK forms an interlocked dimer, with each 80 kDa monomer containing four structural domains. The PPK active site is located in a tunnel, which contains a unique ATP-binding pocket and may accommodate the translocation of synthesized polyP. The PPK structure has laid the foundation for understanding the initiation of polyP synthesis by PPK.

Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis.,Zhu Y, Huang W, Lee SS, Xu W EMBO Rep. 2005 Jul;6(7):681-7. PMID:15947782^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumble KD, Ahn K, Kornberg A. Phosphohistidyl active sites in polyphosphate kinase of Escherichia coli. Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14391-5. PMID:8962061
↑ Zhu Y, Huang W, Lee SS, Xu W. Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis. EMBO Rep. 2005 Jul;6(7):681-7. PMID:15947782

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xdo"

@@ Line 6: / Line 6: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ppk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polyphosphate_kinase Polyphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.1 2.7.4.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xdo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xdo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xdo RCSB], [http://www.ebi.ac.uk/pdbsum/1xdo PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xdo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xdo OCA], [http://pdbe.org/1xdo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xdo RCSB], [http://www.ebi.ac.uk/pdbsum/1xdo PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 28: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1xdo" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

1xdo

From Proteopedia

Revision as of 01:16, 11 September 2015

Crystal Structure of Escherichia coli Polyphosphate Kinase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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