2g0u

From Proteopedia

(Difference between revisions)

Revision as of 03:02, 11 September 2015

Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei

Structural highlights

2g0u is a 1 chain structure with sequence from "bacillus_pseudomallei"_whitmore_1913 "bacillus pseudomallei" whitmore 1913. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Many gram-negative bacteria that are important human pathogens possess type III secretion systems as part of their required virulence factor repertoire. During the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacterial inner and outer membranes and, in many respects, resembles and functions like a syringe. This type III secretion apparatus (TTSA) is used to inject proteins into a host cell's membrane and cytoplasm to subvert normal cellular processes. The external portion of the TTSA is a needle that is composed of a single type of protein that is polymerized in a helical fashion to form an elongated tube with a central channel of 2-3 nm in diameter. TTSA needle proteins from a variety of bacterial pathogens share sequence conservation; however, no atomic structure for any TTSA needle protein is yet available. Here, we report the structure of a TTSA needle protein called BsaL from Burkholderia pseudomallei determined by nuclear magnetic resonance (NMR) spectroscopy. The central part of the protein assumes a helix-turn-helix core domain with two well-defined alpha-helices that are joined by an ordered, four-residue linker. This forms a two-helix bundle that is stabilized by interhelix hydrophobic contacts. Residues that flank this presumably exposed core region are not completely disordered, but adopt a partial helical conformation. The atomic structure of BsaL and its sequence homology with other TTSA needle proteins suggest potentially unique structural dynamics that could be linked with a universal mechanism for control of type III secretion in diverse gram-negative bacterial pathogens.

Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei.,Zhang L, Wang Y, Picking WL, Picking WD, De Guzman RN J Mol Biol. 2006 Jun 2;359(2):322-30. Epub 2006 Mar 30. PMID:16631790^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang L, Wang Y, Picking WL, Picking WD, De Guzman RN. Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei. J Mol Biol. 2006 Jun 2;359(2):322-30. Epub 2006 Mar 30. PMID:16631790 doi:10.1016/j.jmb.2006.03.028

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2g0u"

@@ Line 2: / Line 2: @@
 <StructureSection load='2g0u' size='340' side='right' caption='[[2g0u]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2g0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G0U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2g0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pseudomallei"_whitmore_1913 "bacillus pseudomallei" whitmore 1913]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G0U FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g0u RCSB], [http://www.ebi.ac.uk/pdbsum/2g0u PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0u OCA], [http://pdbe.org/2g0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g0u RCSB], [http://www.ebi.ac.uk/pdbsum/2g0u PDBsum]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 23: / Line 23: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2g0u" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Burkholderia pseudomallei]]
+[[Category: Bacillus pseudomallei whitmore 1913]]
 [[Category: Guzman, R N.De]]
 [[Category: Picking, W D]]

2g0u

From Proteopedia

Revision as of 03:02, 11 September 2015

Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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