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1q5z

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Revision as of 04:19, 11 September 2015

Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)

Structural highlights

1q5z is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[SIPA_SALTY] Actin-binding protein that interferes with host cell actin cytoskeleton. It stimulates actin polymerization and counteracts F-actin destabilizing proteins. Potentiates SipC activity; both are required for an efficient bacterial internalization. In vitro, forms a complex with host cell protein T-plastin increasing actin bundling. It inhibits ADF/cofilin-directed depolymerization both by preventing binding of ADF and cofilin and by displacing them from F-actin. Also protects F-actin from gelsolin-directed severing and reanneals gelsolin-severed F-actin fragments.^[1] ^[2]

Publication Abstract from PubMed

Like many bacterial pathogens, Salmonella spp. use a type III secretion system to inject virulence proteins into host cells. The Salmonella invasion protein A (SipA) binds host actin, enhances its polymerization near adherent extracellular bacteria, and contributes to cytoskeletal rearrangements that internalize the pathogen. By combining x-ray crystallography of SipA with electron microscopy and image analysis of SipA-actin filaments, we show that SipA functions as a "molecular staple," in which a globular domain and two nonglobular "arms" mechanically stabilize the filament by tethering actin subunits in opposing strands. Deletion analysis of the tethering arms provides strong support for this model.

Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms.,Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE Science. 2003 Sep 26;301(5641):1918-21. PMID:14512630^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou D, Mooseker MS, Galan JE. Role of the S. typhimurium actin-binding protein SipA in bacterial internalization. Science. 1999 Mar 26;283(5410):2092-5. PMID:10092234
↑ McGhie EJ, Hayward RD, Koronakis V. Control of actin turnover by a salmonella invasion protein. Mol Cell. 2004 Feb 27;13(4):497-510. PMID:14992720
↑ Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE. Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms. Science. 2003 Sep 26;301(5641):1918-21. PMID:14512630 doi:http://dx.doi.org/10.1126/science.1088433

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1q5z"

@@ Line 2: / Line 2: @@
 <StructureSection load='1q5z' size='340' side='right' caption='[[1q5z]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1q5z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q5Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1q5z]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q5Z FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SipA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5z OCA], [http://pdbe.org/1q5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q5z RCSB], [http://www.ebi.ac.uk/pdbsum/1q5z PDBsum]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q5z RCSB], [http://www.ebi.ac.uk/pdbsum/1q5z PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 15: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1q5z" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
 [[Category: Egelman, E H]]
 [[Category: Galkin, V E]]

1q5z

From Proteopedia

Revision as of 04:19, 11 September 2015

Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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