1g60

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|PDB= 1g60 |SIZE=350|CAPTION= <scene name='initialview01'>1g60</scene>, resolution 1.74&Aring;
|PDB= 1g60 |SIZE=350|CAPTION= <scene name='initialview01'>1g60</scene>, resolution 1.74&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span>
|GENE=
|GENE=
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam01555 N6_N4_Mtase]</span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g60 OCA], [http://www.ebi.ac.uk/pdbsum/1g60 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1g60 RCSB]</span>
}}
}}
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[[Category: Osipiuk, J.]]
[[Category: Osipiuk, J.]]
[[Category: Walsh, M A.]]
[[Category: Walsh, M A.]]
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[[Category: NA]]
 
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[[Category: SAM]]
 
[[Category: dna methylation]]
[[Category: dna methylation]]
[[Category: mcsg]]
[[Category: mcsg]]
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[[Category: structural genomic]]
[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:18:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:52:47 2008''

Revision as of 03:52, 26 March 2008

Image:1g60.gif


PDB ID 1g60

Drag the structure with the mouse to rotate
, resolution 1.74Å
Ligands: ,
Activity: Site-specific DNA-methyltransferase (adenine-specific), with EC number 2.1.1.72
Domains: N6_N4_Mtase
Resources: FirstGlance, OCA, PDBsum, JenaLib, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)


Overview

DNA methyltransferases (MTases) are sequence-specific enzymes which transfer a methyl group from S-adenosyl-L-methionine (AdoMet) to the amino group of either cytosine or adenine within a recognized DNA sequence. Methylation of a base in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing the same DNA sequence. We have determined at 1.74 A resolution the crystal structure of a beta-class DNA MTase MboIIA (M.MboIIA) from the bacterium Moraxella bovis, the smallest DNA MTase determined to date. M.MboIIA methylates the 3' adenine of the pentanucleotide sequence 5'-GAAGA-3'. The protein crystallizes with two molecules in the asymmetric unit which we propose to resemble the dimer when M.MboIIA is not bound to DNA. The overall structure of the enzyme closely resembles that of M.RsrI. However, the cofactor-binding pocket in M.MboIIA forms a closed structure which is in contrast to the open-form structures of other known MTases.

About this Structure

1G60 is a Single protein structure of sequence from Moraxella bovis. Full crystallographic information is available from OCA.

Reference

Crystal structure of MboIIA methyltransferase., Osipiuk J, Walsh MA, Joachimiak A, Nucleic Acids Res. 2003 Sep 15;31(18):5440-8. PMID:12954781

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