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2mjt

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Revision as of 09:50, 11 September 2015

Anoplin R5F T8W in DPC micelles

Structural highlights

2mjt is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	2mjq, 2mjr, 2mjs
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[ANOP_ANOSM] Exhibits potent activity in stimulating degranulation from rat peritoneal mast cells but not from RBL-2H3 cells. Exhibits broad-spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria. Has no hemolytic activity towards human erythrocytes.^[1]

Publication Abstract from PubMed

Antimicrobial peptides (AMPs) are promising candidates for battling multiresistant bacteria. Despite extensive research, structure-activity relationships of AMPs are not fully understood, and there is a lack of structural data relating to AMPs in lipids. Here we present the NMR structure of anoplin (GLLKRIKTLL-NH2 ) in a micellar environment. A vast library of substitutions was designed and tested for antimicrobial and hemolytic activity, as well as for changes in structure and lipid interactions. This showed that improvement of antimicrobial activity without concomitant introduction of strong hemolytic activity can be achieved through subtle increases in the hydrophobicity of the hydrophobic face or through subtle increases in the polarity of the hydrophilic face of the helix, or-most efficiently-a combination of both. A set of guidelines based on the results is given, for assistance in how to modify cationic alpha-helical AMPs in order to control activity and selectivity. The guidelines are finally tested on a different peptide.

Rational Design of Alpha-Helical Antimicrobial Peptides: Do's and Don'ts.,Uggerhoj LE, Poulsen TJ, Munk JK, Fredborg M, Sondergaard TE, Frimodt-Moller N, Hansen PR, Wimmer R Chembiochem. 2015 Jan 19;16(2):242-53. doi: 10.1002/cbic.201402581. Epub 2014 Dec, 21. PMID:25530580^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Konno K, Hisada M, Fontana R, Lorenzi CC, Naoki H, Itagaki Y, Miwa A, Kawai N, Nakata Y, Yasuhara T, Ruggiero Neto J, de Azevedo WF Jr, Palma MS, Nakajima T. Anoplin, a novel antimicrobial peptide from the venom of the solitary wasp Anoplius samariensis. Biochim Biophys Acta. 2001 Nov 26;1550(1):70-80. PMID:11738089
↑ Uggerhoj LE, Poulsen TJ, Munk JK, Fredborg M, Sondergaard TE, Frimodt-Moller N, Hansen PR, Wimmer R. Rational Design of Alpha-Helical Antimicrobial Peptides: Do's and Don'ts. Chembiochem. 2015 Jan 19;16(2):242-53. doi: 10.1002/cbic.201402581. Epub 2014 Dec, 21. PMID:25530580 doi:http://dx.doi.org/10.1002/cbic.201402581

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mjt"

Categories: Poulsen, T J | Uggerhoej, L | Wimmer, R | Amphipathic helix | Antimicrobial protein

@@ Line 6: / Line 6: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2mjq|2mjq]], [[2mjr|2mjr]], [[2mjs|2mjs]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mjt RCSB], [http://www.ebi.ac.uk/pdbsum/2mjt PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjt OCA], [http://pdbe.org/2mjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjt RCSB], [http://www.ebi.ac.uk/pdbsum/2mjt PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2mjt" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

2mjt

From Proteopedia

Revision as of 09:50, 11 September 2015

Anoplin R5F T8W in DPC micelles

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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