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1nr0
From Proteopedia
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|PDB= 1nr0 |SIZE=350|CAPTION= <scene name='initialview01'>1nr0</scene>, resolution 1.70Å | |PDB= 1nr0 |SIZE=350|CAPTION= <scene name='initialview01'>1nr0</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= UNC-78 OR C04F6.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | |GENE= UNC-78 OR C04F6.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00200 WD40], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2319 COG2319]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nr0 OCA], [http://www.ebi.ac.uk/pdbsum/1nr0 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1nr0 RCSB]</span> | ||
}} | }} | ||
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[[Category: Ono, S.]] | [[Category: Ono, S.]] | ||
[[Category: Vorobiev, S M.]] | [[Category: Vorobiev, S M.]] | ||
| - | [[Category: MN]] | ||
[[Category: actin interacting protein]] | [[Category: actin interacting protein]] | ||
[[Category: adf]] | [[Category: adf]] | ||
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[[Category: wd40 repeat]] | [[Category: wd40 repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:59:02 2008'' |
Revision as of 03:59, 26 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | UNC-78 OR C04F6.4 (Caenorhabditis elegans) | ||||||
| Domains: | WD40, COG2319 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Two Seven-Bladed Beta-Propeller Domains Revealed By The Structure Of A C. elegans Homologue Of Yeast Actin Interacting Protein 1 (AIP1).
Overview
Actin-interacting protein 1 (AIP1) is a WD40 repeat protein that enhances actin filament disassembly in the presence of actin-depolymerizing factor (ADF)/cofilin. AIP1 also caps the barbed end of ADF/cofilin-bound actin filament. However, the mechanism by which AIP1 interacts with ADF/cofilin and actin is not clearly understood. We determined the crystal structure of Caenorhabditis elegans AIP1 (UNC-78), which revealed 14 WD40 modules arranged in two seven-bladed beta-propeller domains. The structure allowed for the mapping of conserved surface residues, and mutagenesis studies identified five residues that affected the ADF/cofilin-dependent actin filament disassembly activity. Mutations of these residues, which reside in blades 3 and 4 in the N-terminal propeller domain, had significant effects on the disassembly activity but did not alter the barbed end capping activity. These data support a model in which this conserved surface of AIP1 plays a direct role in enhancing fragmentation/depolymerization of ADF/cofilin-bound actin filaments but not in barbed end capping.
About this Structure
1NR0 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Identification of functional residues on Caenorhabditis elegans actin-interacting protein 1 (UNC-78) for disassembly of actin depolymerizing factor/cofilin-bound actin filaments., Mohri K, Vorobiev S, Fedorov AA, Almo SC, Ono S, J Biol Chem. 2004 Jul 23;279(30):31697-707. Epub 2004 May 18. PMID:15150269
Page seeded by OCA on Wed Mar 26 05:59:02 2008
Categories: Caenorhabditis elegans | Single protein | Almo, S C. | Burley, S K. | Mohri, K. | NYSGXRC, New York Structural GenomiX Research Consortium. | Ono, S. | Vorobiev, S M. | Actin interacting protein | Adf | Beta propeller | Cofilin | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomic | Wd40 repeat
