1ox9

From Proteopedia

(Difference between revisions)

Revision as of 12:38, 11 September 2015

Crystal structure of SspB-ssrA complex

Structural highlights

1ox9 is a 16 chain structure with sequence from Eco57. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1ox8
Gene:	SSPB OR B3228 OR Z4586 OR ECS4101 (ECO57)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[SSPB_ECOLI] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.^[1] ^[2] ^[3] Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion.^[4] ^[5] ^[6]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates.

Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine.,Song HK, Eck MJ Mol Cell. 2003 Jul;12(1):75-86. PMID:12887894^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Levchenko I, Seidel M, Sauer RT, Baker TA. A specificity-enhancing factor for the ClpXP degradation machine. Science. 2000 Sep 29;289(5488):2354-6. PMID:11009422
↑ Flynn JM, Levchenko I, Sauer RT, Baker TA. Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradation. Genes Dev. 2004 Sep 15;18(18):2292-301. PMID:15371343 doi:http://dx.doi.org/10.1101/gad.1240104
↑ Song HK, Eck MJ. Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine. Mol Cell. 2003 Jul;12(1):75-86. PMID:12887894
↑ Levchenko I, Seidel M, Sauer RT, Baker TA. A specificity-enhancing factor for the ClpXP degradation machine. Science. 2000 Sep 29;289(5488):2354-6. PMID:11009422
↑ Flynn JM, Levchenko I, Sauer RT, Baker TA. Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradation. Genes Dev. 2004 Sep 15;18(18):2292-301. PMID:15371343 doi:http://dx.doi.org/10.1101/gad.1240104
↑ Song HK, Eck MJ. Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine. Mol Cell. 2003 Jul;12(1):75-86. PMID:12887894
↑ Song HK, Eck MJ. Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine. Mol Cell. 2003 Jul;12(1):75-86. PMID:12887894

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ox9"

Categories: Eco57 | Eck, M J | Song, H K | Hydrolase activator | Sspb-ssra

@@ Line 2: / Line 2: @@
 <StructureSection load='1ox9' size='340' side='right' caption='[[1ox9]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ox9]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OX9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ox9]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OX9 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ox8|1ox8]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSPB OR B3228 OR Z4586 OR ECS4101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSPB OR B3228 OR Z4586 OR ECS4101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ox9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ox9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ox9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ox9 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ox9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ox9 OCA], [http://pdbe.org/1ox9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ox9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ox9 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1ox9" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Escherichia coli]]
+[[Category: Eco57]]
 [[Category: Eck, M J]]
 [[Category: Song, H K]]
 [[Category: Hydrolase activator]]
 [[Category: Sspb-ssra]]

1ox9

From Proteopedia

Revision as of 12:38, 11 September 2015

Crystal structure of SspB-ssrA complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox