2g0l

From Proteopedia

(Difference between revisions)

Revision as of 13:53, 11 September 2015

Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone

Structural highlights

2g0l is a 1 chain structure with sequence from 'streptomyces carzinostaticus'. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1j5h, 1j5i, 2g0k
Gene:	ncsA ('Streptomyces carzinostaticus')
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[NCZS_STRCZ] NCS has antibiotic activity (for Gram-positive bacteria) and antitumor activity (for certain mouse tumors). NCS binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.

Publication Abstract from PubMed

Neocarzinostatin (NCS) is a 1:1 complex of an enediyne chromophore (NCSChrom), non-covalently bound to an 11 kDa protein (apoNCS). We are exploring apoNCS as a generic protein system for sequestering small molecules for therapeutic applications. Here, we disclose a new flavone ligand 1 for apoNCS and present a high-resolution NMR structure of this ligand bound to apoNCS. This is the first high-resolution structure of a completely non-cognate ligand bound to the apoNCS protein. This work provides unambiguous evidence that a completely new class of ligand can bind specifically to apoNCS. Furthermore, the mode of binding is different than that of the naphthoate-based ligands, and for such a simple hydrophobic compound, the new ligand surprisingly binds specifically. This work indicates that apo-Neocarzinostatin has multiple selective and distinct binding modes for small-molecule cargo.

Synthetic ligands for apo-neocarzinostatin.,Caddick S, Muskett FW, Stoneman RG, Woolfson DN J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:16568976^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Caddick S, Muskett FW, Stoneman RG, Woolfson DN. Synthetic ligands for apo-neocarzinostatin. J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:16568976 doi:10.1021/ja058419i

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2g0l"

@@ Line 2: / Line 2: @@
 <StructureSection load='2g0l' size='340' side='right' caption='[[2g0l]], [[NMR_Ensembles_of_Models | 58 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2g0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G0L FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2g0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_carzinostaticus' 'streptomyces carzinostaticus']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G0L FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLN:2-PHENYL-4H-CHROMEN-4-ONE'>FLN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1j5h|1j5h]], [[1j5i|1j5i]], [[2g0k|2g0k]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ncsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1897 Streptomyces carzinostaticus])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ncsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1897 'Streptomyces carzinostaticus'])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g0l RCSB], [http://www.ebi.ac.uk/pdbsum/2g0l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0l OCA], [http://pdbe.org/2g0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g0l RCSB], [http://www.ebi.ac.uk/pdbsum/2g0l PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2g0l" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

2g0l

From Proteopedia

Revision as of 13:53, 11 September 2015

Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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