1u8x
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1u8x |SIZE=350|CAPTION= <scene name='initialview01'>1u8x</scene>, resolution 2.05Å | |PDB= 1u8x |SIZE=350|CAPTION= <scene name='initialview01'>1u8x</scene>, resolution 2.05Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122] </span> |
|GENE= glvA, glvG, glv-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |GENE= glvA, glvG, glv-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02056 Glyco_hydro_4]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8x OCA], [http://www.ebi.ac.uk/pdbsum/1u8x PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1u8x RCSB]</span> | ||
}} | }} | ||
| Line 28: | Line 30: | ||
[[Category: Rajan, S S.]] | [[Category: Rajan, S S.]] | ||
[[Category: Yang, X.]] | [[Category: Yang, X.]] | ||
| - | [[Category: G6P]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: NAD]] | ||
[[Category: glucosidase]] | [[Category: glucosidase]] | ||
[[Category: mcsg]] | [[Category: mcsg]] | ||
| Line 39: | Line 38: | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:09:14 2008'' |
Revision as of 04:09, 26 March 2008
| |||||||
| , resolution 2.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | glvA, glvG, glv-1 (Bacillus subtilis) | ||||||
| Activity: | Maltose-6'-phosphate glucosidase, with EC number 3.2.1.122 | ||||||
| Domains: | Glyco_hydro_4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE
Overview
GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 A resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal.
About this Structure
1U8X is a Single protein structure of sequence from Bacillus subtilis. This structure supersedes the now removed PDB entry 1NRH. Full crystallographic information is available from OCA.
Reference
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:15341727
Page seeded by OCA on Wed Mar 26 06:09:14 2008
Categories: Bacillus subtilis | Maltose-6'-phosphate glucosidase | Single protein | Anderson, W F. | Collart, F. | MCSG, Midwest Center for Structural Genomics. | Rajan, S S. | Yang, X. | Glucosidase | Mcsg | Midwest center for structural genomic | Nad-dependent | Protein structure initiative | Psi | Structural genomic
