2ljc

From Proteopedia

(Difference between revisions)

Revision as of 15:19, 11 September 2015

Structure of the influenza AM2-BM2 chimeric channel bound to rimantadine

Structural highlights

2ljc is a 4 chain structure with sequence from Influenza a virus h3n2. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2rlf, 2kix, 2ljb, 2kwx
Gene:	BM2 (Influenza A virus H3N2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[Q9YP62_9INFA] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).[SAAS:SAAS002089_004_400258]

Publication Abstract from PubMed

The M2 channel of influenza A is a target of the adamantane family antiviral drugs. Two different drug-binding sites have been reported: one inside the pore, and the other is a lipid-facing pocket. A previous study showed that a chimera of M2 variants from influenza A and B that contains only the pore-binding site is sensitive to amantadine inhibition, suggesting that the primary site of inhibition is inside the pore. To obtain atomic details of channel-drug interaction, we determined the structures of the chimeric channel with and without rimantadine. Inside the channel and near the N-terminal end, methyl groups of Val27 and Ala30 from four subunits form a hydrophobic pocket around the adamantane, and the drug amino group appears to be in polar contact with the backbone oxygen of Ala30. The structures also reveal differences between the drug-bound and -unbound states of the channel that can explain drug resistance.

Structural investigation of rimantadine inhibition of the AM2-BM2 chimera channel of influenza viruses.,Pielak RM, Oxenoid K, Chou JJ Structure. 2011 Nov 9;19(11):1655-63. PMID:22078564^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pielak RM, Oxenoid K, Chou JJ. Structural investigation of rimantadine inhibition of the AM2-BM2 chimera channel of influenza viruses. Structure. 2011 Nov 9;19(11):1655-63. PMID:22078564 doi:10.1016/j.str.2011.09.003

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ljc"

@@ Line 2: / Line 2: @@
 <StructureSection load='2ljc' size='340' side='right' caption='[[2ljc]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ljc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_b_virus_(b/taiwan/70061/2006) Influenza b virus (b/taiwan/70061/2006)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LJC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ljc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus_h3n2 Influenza a virus h3n2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LJC FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RIM:RIMANTADINE'>RIM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2rlf|2rlf]], [[2kix|2kix]], [[2ljb|2ljb]], [[2kwx|2kwx]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=529646 Influenza B virus (B/Taiwan/70061/2006)])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=41857 Influenza A virus H3N2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ljc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ljc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ljc RCSB], [http://www.ebi.ac.uk/pdbsum/2ljc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ljc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ljc OCA], [http://pdbe.org/2ljc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ljc RCSB], [http://www.ebi.ac.uk/pdbsum/2ljc PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2ljc" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Influenza a virus h3n2]]
 [[Category: Chou, J J]]
 [[Category: Oxenoid, K]]

2ljc

From Proteopedia

Revision as of 15:19, 11 September 2015

Structure of the influenza AM2-BM2 chimeric channel bound to rimantadine

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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