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2gjf

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Revision as of 17:10, 11 September 2015

NMR structure of the computationally designed procarboxypeptidase-A (1AYE) domain

Structural highlights

2gjf is a 2 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1vjq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Recent efforts to design de novo or redesign the sequence and structure of proteins using computational techniques have met with significant success. Most, if not all, of these computational methodologies attempt to model atomic-level interactions, and hence high-resolution structural characterization of the designed proteins is critical for evaluating the atomic-level accuracy of the underlying design force-fields. We previously used our computational protein design protocol RosettaDesign to completely redesign the sequence of the activation domain of human procarboxypeptidase A2. With 68% of the wild-type sequence changed, the designed protein, AYEdesign, is over 10 kcal/mol more stable than the wild-type protein. Here, we describe the high-resolution crystal structure and solution NMR structure of AYEdesign, which show that the experimentally determined backbone and side-chains conformations are effectively superimposable with the computational model at atomic resolution. To isolate the origins of the remarkable stabilization, we have designed and characterized a new series of procarboxypeptidase mutants that gain significant thermodynamic stability with a minimal number of mutations; one mutant gains more than 5 kcal/mol of stability over the wild-type protein with only four amino acid changes. We explore the relationship between force-field smoothing and conformational sampling by comparing the experimentally determined free energies of the overall design and these focused subsets of mutations to those predicted using modified force-fields, and both fixed and flexible backbone sampling protocols.

High-resolution structural and thermodynamic analysis of extreme stabilization of human procarboxypeptidase by computational protein design.,Dantas G, Corrent C, Reichow SL, Havranek JJ, Eletr ZM, Isern NG, Kuhlman B, Varani G, Merritt EA, Baker D J Mol Biol. 2007 Mar 2;366(4):1209-21. Epub 2006 Dec 2. PMID:17196978^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dantas G, Corrent C, Reichow SL, Havranek JJ, Eletr ZM, Isern NG, Kuhlman B, Varani G, Merritt EA, Baker D. High-resolution structural and thermodynamic analysis of extreme stabilization of human procarboxypeptidase by computational protein design. J Mol Biol. 2007 Mar 2;366(4):1209-21. Epub 2006 Dec 2. PMID:17196978 doi:10.1016/j.jmb.2006.11.080

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gjf"

Categories: Reichow, S | De novo protein | Designed protein | Procarboxypeptidase

@@ Line 4: / Line 4: @@
 <table><tr><td colspan='2'>[[2gjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GJF FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1vjq|1vjq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gjf RCSB], [http://www.ebi.ac.uk/pdbsum/2gjf PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjf OCA], [http://pdbe.org/2gjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gjf RCSB], [http://www.ebi.ac.uk/pdbsum/2gjf PDBsum]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 24: / Line 24: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2gjf" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

2gjf

From Proteopedia

Revision as of 17:10, 11 September 2015

NMR structure of the computationally designed procarboxypeptidase-A (1AYE) domain

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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