2i9l

From Proteopedia

(Difference between revisions)

Revision as of 17:55, 11 September 2015

Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein

Structural highlights

2i9l is a 12 chain structure with sequence from Mus musculus and Vaccinia virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	l1r (Vaccinia virus)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[L1_VACCW] Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Medical countermeasures to prevent or treat smallpox are needed due to the potential use of poxviruses as biological weapons. Safety concerns with the currently available smallpox vaccine indicate a need for research on alternative poxvirus vaccine strategies. Molecular vaccines involving the use of proteins and/or genes and recombinant antibodies are among the strategies under current investigation. The poxvirus L1 protein, encoded by the L1R open reading frame, is the target of neutralizing antibodies and has been successfully used as a component of both protein subunit and DNA vaccines. L1-specific monoclonal antibodies (e.g., mouse monoclonal antibody mAb-7D11, mAb-10F5) with potent neutralizing activity bind L1 in a conformation-specific manner. This suggests that proper folding of the L1 protein used in molecular vaccines will affect the production of neutralizing antibodies and protection. Here, we co-crystallized the Fab fragment of mAb-7D11 with the L1 protein. The crystal structure of the complex between Fab-7D11 and L1 reveals the basis for the conformation-specific binding as recognition of a discontinuous epitope containing two loops that are held together by a disulfide bond. The structure of this important conformational epitope of L1 will contribute to the development of molecular poxvirus vaccines and also provides a novel target for anti-poxvirus drugs. In addition, the sequence and structure of Fab-7D11 will contribute to the development of L1-targeted immunotherapeutics.

Structural basis for the binding of the neutralizing antibody, 7D11, to the poxvirus L1 protein.,Su HP, Golden JW, Gittis AG, Hooper JW, Garboczi DN Virology. 2007 Nov 25;368(2):331-41. Epub 2007 Aug 3. PMID:17688903^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ravanello MP, Hruby DE. Conditional lethal expression of the vaccinia virus L1R myristylated protein reveals a role in virion assembly. J Virol. 1994 Oct;68(10):6401-10. PMID:8083978
↑ Bisht H, Weisberg AS, Moss B. Vaccinia virus l1 protein is required for cell entry and membrane fusion. J Virol. 2008 Sep;82(17):8687-94. doi: 10.1128/JVI.00852-08. Epub 2008 Jul 2. PMID:18596103 doi:http://dx.doi.org/10.1128/JVI.00852-08
↑ Foo CH, Lou H, Whitbeck JC, Ponce-de-Leon M, Atanasiu D, Eisenberg RJ, Cohen GH. Vaccinia virus L1 binds to cell surfaces and blocks virus entry independently of glycosaminoglycans. Virology. 2009 Mar 15;385(2):368-82. doi: 10.1016/j.virol.2008.12.019. Epub 2009 , Jan 21. PMID:19162289 doi:http://dx.doi.org/10.1016/j.virol.2008.12.019
↑ Su HP, Golden JW, Gittis AG, Hooper JW, Garboczi DN. Structural basis for the binding of the neutralizing antibody, 7D11, to the poxvirus L1 protein. Virology. 2007 Nov 25;368(2):331-41. Epub 2007 Aug 3. PMID:17688903 doi:10.1016/j.virol.2007.06.042

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i9l"

@@ Line 5: / Line 5: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">l1r ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10245 Vaccinia virus])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i9l RCSB], [http://www.ebi.ac.uk/pdbsum/2i9l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9l OCA], [http://pdbe.org/2i9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i9l RCSB], [http://www.ebi.ac.uk/pdbsum/2i9l PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VM25_VACCV VM25_VACCV]] Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.<ref>PMID:8083978</ref> <ref>PMID:18596103</ref> <ref>PMID:19162289</ref>
+[[http://www.uniprot.org/uniprot/L1_VACCW L1_VACCW]] Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.<ref>PMID:8083978</ref> <ref>PMID:18596103</ref> <ref>PMID:19162289</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2i9l" style="background-color:#fffaf0;"></div>
 ==See Also==

2i9l

From Proteopedia

Revision as of 17:55, 11 September 2015

Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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