1g71

From Proteopedia

(Difference between revisions)

Revision as of 23:03, 11 September 2015

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

Structural highlights

1g71 is a 2 chain structure with sequence from Atcc 43587. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	1dd9, 1dde, 1eqn
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[PRIS_PYRFU] Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Primases are essential components of the DNA replication apparatus in every organism. They catalyze the synthesis of oligoribonucleotides on single-stranded DNA, which subsequently serve as primers for the replicative DNA polymerases. In contrast to bacterial primases, the archaeal enzymes are closely related to their eukaryotic counterparts. We have solved the crystal structure of the catalytic primase subunit from the hyperthermophilic archaeon Pyrococcus furiosus at 2.3 A resolution by multiwavelength anomalous dispersion methods. The structure shows a two-domain arrangement with a novel zinc knuckle motif located in the primase (prim) domain. In this first structure of a complete protein of the archaeal/eukaryotic primase family, the arrangement of the catalytically active residues resembles the active sites of various DNA polymerases that are unrelated in fold.

Crystal structure of a DNA-dependent RNA polymerase (DNA primase).,Augustin MA, Huber R, Kaiser JT Nat Struct Biol. 2001 Jan;8(1):57-61. PMID:11135672^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu L, Komori K, Ishino S, Bocquier AA, Cann IK, Kohda D, Ishino Y. The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus. J Biol Chem. 2001 Nov 30;276(48):45484-90. Epub 2001 Oct 2. PMID:11584001 doi:http://dx.doi.org/10.1074/jbc.M106391200
↑ Augustin MA, Huber R, Kaiser JT. Crystal structure of a DNA-dependent RNA polymerase (DNA primase). Nat Struct Biol. 2001 Jan;8(1):57-61. PMID:11135672 doi:10.1038/83060

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g71"

@@ Line 2: / Line 2: @@
 <StructureSection load='1g71' size='340' side='right' caption='[[1g71]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1g71]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G71 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1g71]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G71 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dd9|1dd9]], [[1dde|1dde]], [[1eqn|1eqn]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g71 RCSB], [http://www.ebi.ac.uk/pdbsum/1g71 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g71 OCA], [http://pdbe.org/1g71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g71 RCSB], [http://www.ebi.ac.uk/pdbsum/1g71 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1g71" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 34: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Pyrococcus furiosus]]
+[[Category: Atcc 43587]]
 [[Category: Augustin, M A]]
 [[Category: Huber, R]]

1g71

From Proteopedia

Revision as of 23:03, 11 September 2015

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox