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4xgr

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Revision as of 07:45, 30 September 2015

Crystal structure of addiction module from Mycobacterial species

Structural highlights

4xgr is a 8 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	4xgq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[VPC30_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB30.[HAMAP-Rule:MF_00265]^[1] [VPB30_MYCTU] Antitoxin component of a type II toxin-antitoxin (TA) module. Upon expression in M.smegmatis neutralizes the effect of cognate toxin VapC30.

Publication Abstract from PubMed

Toxin-antitoxin (TA) systems play important roles in bacterial physiology, such as multidrug tolerance, biofilm formation, and arrest of cellular growth under stress conditions. To develop novel antimicrobial agents against tuberculosis, we focused on VapBC systems, which encompass more than half of TA systems in Mycobacterium tuberculosis. Here, we report that theMycobacterium tuberculosis VapC30 toxin regulates cellular growth through both magnesium and manganese ion-dependent ribonuclease activity and is inhibited by the cognate VapB30 antitoxin. We also determined the 2.7-A resolution crystal structure of the M. tuberculosis VapBC30 complex, which revealed a novel process of inactivation of the VapC30 toxin via swapped blocking by the VapB30 antitoxin. Our study on M. tuberculosis VapBC30 leads us to design two kinds of VapB30 and VapC30-based novel peptides which successfully disrupt the toxin-antitoxin complex and thus activate the ribonuclease activity of the VapC30 toxin. Our discovery herein possibly paves the way to treat tuberculosis for next generation.

Structural and functional studies of the Mycobacterium tuberculosis VapBC30 toxin-antitoxin system: implications for the design of novel antimicrobial peptides.,Lee IG, Lee SJ, Chae S, Lee KY, Kim JH, Lee BJ Nucleic Acids Res. 2015 Jul 6. pii: gkv689. PMID:26150422^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ramage HR, Connolly LE, Cox JS. Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009 Dec;5(12):e1000767. doi: 10.1371/journal.pgen.1000767. Epub 2009, Dec 11. PMID:20011113 doi:http://dx.doi.org/10.1371/journal.pgen.1000767
↑ Lee IG, Lee SJ, Chae S, Lee KY, Kim JH, Lee BJ. Structural and functional studies of the Mycobacterium tuberculosis VapBC30 toxin-antitoxin system: implications for the design of novel antimicrobial peptides. Nucleic Acids Res. 2015 Jul 6. pii: gkv689. PMID:26150422 doi:http://dx.doi.org/10.1093/nar/gkv689

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xgr"

@@ Line 6: / Line 6: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xgq|4xgq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xgr RCSB], [http://www.ebi.ac.uk/pdbsum/4xgr PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgr OCA], [http://pdbe.org/4xgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xgr RCSB], [http://www.ebi.ac.uk/pdbsum/4xgr PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4xgr" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4xgr

From Proteopedia

Revision as of 07:45, 30 September 2015

Crystal structure of addiction module from Mycobacterial species

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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