2ns1

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|PDB= 2ns1 |SIZE=350|CAPTION= <scene name='initialview01'>2ns1</scene>, resolution 1.962&Aring;
|PDB= 2ns1 |SIZE=350|CAPTION= <scene name='initialview01'>2ns1</scene>, resolution 1.962&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), glnK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), glnK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK10666 PRK10666], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK10665 PRK10665]</span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ns1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns1 OCA], [http://www.ebi.ac.uk/pdbsum/2ns1 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2ns1 RCSB]</span>
}}
}}
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[[Category: III, J O.Connell.]]
[[Category: III, J O.Connell.]]
[[Category: Stroud, R M.]]
[[Category: Stroud, R M.]]
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[[Category: ADP]]
 
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[[Category: BOG]]
 
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[[Category: TRS]]
 
[[Category: adp]]
[[Category: adp]]
[[Category: ammonia]]
[[Category: ammonia]]
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[[Category: structural genomic]]
[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:28:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:34:48 2008''

Revision as of 04:34, 26 March 2008

Image:2ns1.jpg


PDB ID 2ns1

Drag the structure with the mouse to rotate
, resolution 1.962Å
Ligands: , ,
Gene: amtB (Escherichia coli), glnK (Escherichia coli)
Domains: PRK10666, PRK10665
Resources: FirstGlance, OCA, PDBsum, JenaLib, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK


Overview

Ammonia conductance is highly regulated. A P(II) signal transduction protein, GlnK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GlnK at 1.96-A resolution shows that the trimeric channel is blocked directly by GlnK and how, in response to intracellular nitrogen status, the ability of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.

About this Structure

2NS1 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A., Gruswitz F, O'Connell J 3rd, Stroud RM, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:17190799

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