Cas9 Sandbox

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	* any?		* any?
-	<scene name='71/714945/Pam_interaction/8'>TextToBeDisplayed</scene>	+	<scene name='71/714945/Pam_interaction/8'>Cas9 interaction with PAM >
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-	==3D structures of Cas9==	+
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-	===''Streptococcus pyogenes'' Cas9===	+
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-	* [[4un3]], [[4un4]], and [[4un5]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA	+
-	[[Image:4un3 labeled.png|right|390px]]	+
-	* [[4oo8]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA	+
-	* [[4cmp]]	+
-	* [[4cmq]] - Mn<sup>2+</sup>-bound ''S. pyogenes'' Cas9	+
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-	=== ''Actinomyces naeslundii'' Cas9===	+
-	*[[4oge]]	+
-	*[[4ogc]] - Mn<sup>2+</sup>-bound ''A.s naeslundii'' Cas9	+
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-	==See Also==	+
-	[[HNH endonuclease]] <br/>	+
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-	== References ==	+
-	<references/>	+
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-	[[Category: Crispr]]	+
-	[[Category: Crispr-associated]]	+
-	[[Category: endonuclease]]	+
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-	The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.	+
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-	[[Image:Layout for schematic and structure with structure.png|660px]]	+
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-	Articles in Proteopedia concerning Cas9 include:	+
-	* any?	+
	==3D structures of Cas9==		==3D structures of Cas9==

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Revision as of 14:22, 6 October 2015

Cas9 is the RNA-guided DNA endonuclease used by the CRISPR (clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.

spinqualitylabelsall models Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.

Articles in Proteopedia concerning Cas9 include:

• any?