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| - | ==Hybrid structure of the Type 1 Pilus of Uropathogenic E.coli==
| + | #REDIRECT [[2n7h]] This PDB entry is obsolete and replaced by 2n7h |
| - | <StructureSection load='2mx3' size='340' side='right' caption='[[2mx3]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[2mx3]] is a 6 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MX3 FirstGlance]. <br>
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| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mx3 RCSB], [http://www.ebi.ac.uk/pdbsum/2mx3 PDBsum]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [[http://www.uniprot.org/uniprot/FIMA1_ECOLI FIMA1_ECOLI]] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Type 1 pili are filamentous protein assemblies on the surface of Gram-negative bacteria that mediate adhesion to host cells during the infection process. The molecular structure of type 1 pili remains elusive on the atomic scale owing to their insolubility and noncrystallinity. Herein we describe an approach for hybrid-structure determination that is based on data from solution-state NMR spectroscopy on the soluble subunit and solid-state NMR spectroscopy and STEM data on the assembled pilus. Our approach is based on iterative modeling driven by structural information extracted from different sources and provides a general tool to access pseudo atomic structures of protein assemblies with complex subunit folds. By using this methodology, we determined the local conformation of the FimA pilus subunit in the context of the assembled type 1 pilus, determined the exact helical pilus architecture, and elucidated the intermolecular interfaces contributing to pilus assembly and stability with atomic detail.
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| - | Hybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli.,Habenstein B, Loquet A, Hwang S, Giller K, Vasa SK, Becker S, Habeck M, Lange A Angew Chem Int Ed Engl. 2015 Aug 12. doi: 10.1002/anie.201505065. PMID:26267365<ref>PMID:26267365</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Becker, S]]
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| - | [[Category: Giller, K]]
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| - | [[Category: Habeck, M]]
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| - | [[Category: Habenstein, B]]
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| - | [[Category: Lange, A]]
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| - | [[Category: Loquet, A]]
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| - | [[Category: Vasa, S]]
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| - | [[Category: Structural protein]]
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