2qmo
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2qmo |SIZE=350|CAPTION= <scene name='initialview01'>2qmo</scene>, resolution 1.47Å | |PDB= 2qmo |SIZE=350|CAPTION= <scene name='initialview01'>2qmo</scene>, resolution 1.47Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Dethiobiotin_synthase Dethiobiotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.3 6.3.3.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dethiobiotin_synthase Dethiobiotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.3 6.3.3.3] </span> |
|GENE= bioD, HP_0029 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Helicobacter pylori 26695]) | |GENE= bioD, HP_0029 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Helicobacter pylori 26695]) | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0132 BioD], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd03109 DTBS]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qmo OCA], [http://www.ebi.ac.uk/pdbsum/2qmo PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2qmo RCSB]</span> | ||
}} | }} | ||
| Line 33: | Line 35: | ||
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
[[Category: Zheng, H.]] | [[Category: Zheng, H.]] | ||
| - | [[Category: CL]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: biotin biosynthesis]] | [[Category: biotin biosynthesis]] | ||
| Line 46: | Line 47: | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:43:25 2008'' |
Revision as of 04:43, 26 March 2008
| |||||||
| , resolution 1.47Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | bioD, HP_0029 (Helicobacter pylori 26695) | ||||||
| Activity: | Dethiobiotin synthase, with EC number 6.3.3.3 | ||||||
| Domains: | BioD, DTBS | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of dethiobiotin synthetase (bioD) from Helicobacter pylori
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2QMO is a Single protein structure of sequence from Helicobacter pylori 26695. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Wed Mar 26 06:43:25 2008
Categories: Dethiobiotin synthase | Helicobacter pylori 26695 | Single protein | Chruszcz, M. | Cuff, M. | Cymborowski, M. | Edwards, A. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Minor, W. | Savchenko, A. | Xu, X. | Zheng, H. | Atp-binding | Biotin biosynthesis | Dethiobiotin synthetase | Ligase | Magnesium | Mcsg | Midwest center for structural genomic | Nucleotide-binding | Protein structure initiative | Psi-2 | Structural genomic
