2jzz
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(New page: 200px {{Structure |PDB= 2jzz |SIZE=350|CAPTION= <scene name='initialview01'>2jzz</scene> |SITE= |LIGAND= |ACTIVITY= |GENE= |DOMAIN=<span class='plainlinks'>[h...)
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Revision as of 07:51, 26 March 2008
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| Domains: | Ubiquitin | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solid-State NMR Structure of Microcrystalline Ubiquitin
Overview
Proton-driven 13C spin diffusion (PDSD) is a simple and robust two-dimensional NMR experiment. It leads to spectra with a high signal-to-noise ratio in which cross-peaks contain information about internuclear distances. We show that the total information content is sufficient to determine the atomic-resolution structure of a small protein from a single, uniformly 13C-, 15N-labeled microcrystalline sample. For the example of ubiquitin, the structure was determined by a manual procedure followed by an automatic optimization of the manual structure as well as by a fully automated structure determination approach. The relationship between internuclear distances and cross-peak intensities in the spectra is investigated.
About this Structure
2JZZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy., Manolikas T, Herrmann T, Meier BH, J Am Chem Soc. 2008 Mar 26;130(12):3959-66. Epub 2008 Mar 6. PMID:18321098
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