Barnase
From Proteopedia
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== Function == | == Function == | ||
| - | '''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. | + | '''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. |
== Relevance == | == Relevance == | ||
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== Structural highlights == | == Structural highlights == | ||
| - | BA hydrolyzes RNA at GpN sites where the active site positively charged Lys and Arg coordinate the RNA phosphate group. | + | BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/3'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene>.<ref>PMID:8110767</ref> |
</StructureSection> | </StructureSection> | ||
Revision as of 11:13, 9 November 2015
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3D Structures of Barnase
Updated on 09-November-2015
References
- ↑ Buckle AM, Fersht AR. Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Biochemistry. 1994 Feb 22;33(7):1644-53. PMID:8110767
