2rhb
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= NSP15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 SARS coronavirus]) | |GENE= NSP15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 SARS coronavirus]) | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam06471 NSP11]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rhb OCA], [http://www.ebi.ac.uk/pdbsum/2rhb PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2rhb RCSB]</span> | ||
}} | }} | ||
'''Crystal structure of Nsp15-H234A mutant- Hexamer in asymmetric unit''' | '''Crystal structure of Nsp15-H234A mutant- Hexamer in asymmetric unit''' | ||
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| + | ==Overview== | ||
| + | The severe acute respiratory syndrome (SARS) coronavirus encodes several RNA-processing enzymes that are unusual for RNA viruses, including Nsp15 (nonstructural protein 15), a hexameric endoribonuclease that preferentially cleaves 3' of uridines. We solved the structure of a catalytically inactive mutant version of Nsp15, which was crystallized as a hexamer. The structure contains unreported flexibility in the active site of each subunit. Substitutions in the active site residues serine 293 and proline 343 allowed Nsp15 to cleave at cytidylate, whereas mutation of leucine 345 rendered Nsp15 able to cleave at purines as well as pyrimidines. Mutations that targeted the residues involved in subunit interactions generally resulted in the formation of catalytically inactive monomers. The RNA-binding residues were mapped by a method linking reversible cross-linking, RNA affinity purification, and peptide fingerprinting. Alanine substitution of several residues in the RNA-contacting portion of Nsp15 did not affect hexamer formation but decreased the affinity of RNA binding and reduced endonuclease activity. This suggests a model for Nsp15 hexamer interaction with RNA. | ||
==About this Structure== | ==About this Structure== | ||
2RHB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RHB OCA]. | 2RHB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RHB OCA]. | ||
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| + | ==Reference== | ||
| + | Structural and functional analyses of the severe acute respiratory syndrome coronavirus endoribonuclease Nsp15., Bhardwaj K, Palaninathan S, Alcantara JM, Yi LL, Guarino L, Sacchettini JC, Kao CC, J Biol Chem. 2008 Feb 8;283(6):3655-64. Epub 2007 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18045871 18045871] | ||
[[Category: Sars coronavirus]] | [[Category: Sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: viral protein]] | [[Category: viral protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 10:03:05 2008'' |
Revision as of 08:03, 26 March 2008
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| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | NSP15 (SARS coronavirus) | ||||||
| Domains: | NSP11 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Nsp15-H234A mutant- Hexamer in asymmetric unit
Overview
The severe acute respiratory syndrome (SARS) coronavirus encodes several RNA-processing enzymes that are unusual for RNA viruses, including Nsp15 (nonstructural protein 15), a hexameric endoribonuclease that preferentially cleaves 3' of uridines. We solved the structure of a catalytically inactive mutant version of Nsp15, which was crystallized as a hexamer. The structure contains unreported flexibility in the active site of each subunit. Substitutions in the active site residues serine 293 and proline 343 allowed Nsp15 to cleave at cytidylate, whereas mutation of leucine 345 rendered Nsp15 able to cleave at purines as well as pyrimidines. Mutations that targeted the residues involved in subunit interactions generally resulted in the formation of catalytically inactive monomers. The RNA-binding residues were mapped by a method linking reversible cross-linking, RNA affinity purification, and peptide fingerprinting. Alanine substitution of several residues in the RNA-contacting portion of Nsp15 did not affect hexamer formation but decreased the affinity of RNA binding and reduced endonuclease activity. This suggests a model for Nsp15 hexamer interaction with RNA.
About this Structure
2RHB is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.
Reference
Structural and functional analyses of the severe acute respiratory syndrome coronavirus endoribonuclease Nsp15., Bhardwaj K, Palaninathan S, Alcantara JM, Yi LL, Guarino L, Sacchettini JC, Kao CC, J Biol Chem. 2008 Feb 8;283(6):3655-64. Epub 2007 Nov 28. PMID:18045871
Page seeded by OCA on Wed Mar 26 10:03:05 2008
Categories: Sars coronavirus | Single protein | Alcantara, J M.O. | Bhardwaj, K. | Guarino, L. | Kao, C C. | Palaninathan, S. | Sacchettini, J. | Yi, L L. | Endoribonuclease | Hexamer | Nsp | Rna | Sar | Viral protein
