193l
From Proteopedia
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|PDB= 193l |SIZE=350|CAPTION= <scene name='initialview01'>193l</scene>, resolution 1.33Å | |PDB= 193l |SIZE=350|CAPTION= <scene name='initialview01'>193l</scene>, resolution 1.33Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=193l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=193l OCA], [http://www.ebi.ac.uk/pdbsum/193l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=193l RCSB]</span> | ||
}} | }} | ||
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[[Category: Ries-Kautt, M.]] | [[Category: Ries-Kautt, M.]] | ||
[[Category: Vaney, M C.]] | [[Category: Vaney, M C.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: NA]] | ||
[[Category: hydrolase (o-glycosyl)]] | [[Category: hydrolase (o-glycosyl)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:29:57 2008'' |
Revision as of 15:30, 30 March 2008
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| , resolution 1.33Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE 1.33 A STRUCTURE OF TETRAGONAL HEN EGG WHITE LYSOZYME
Overview
Crystals of tetragonal hen egg-white lysozyme were grown using Advanced Protein Crystallization Facility (APCF) apparatus under a microgravity environment (SpaceHab-01 mission) and ground control conditions. Crystals were grown from NaCl as a crystallizing agent at pH 4.3. The X-ray diffraction patterns of the best diffracting ground- and space-grown crystals were recorded using synchrotron radiation and an image plate on the W32 beamline at LURE. Both ground- and space-grown crystals showed nearly equivalent maximum resolution of 1.3-1.4 A. Refinements were carried out with the program X-PLOR with final R values of 18.45 and 18.27% for structures from ground- and space- grown crystals, respectively. The two structures are nearly identical with the root-mean-square difference on all protein atoms being 0.13 A. Some residues of the two refined structures show multiple alternative conformations. Two ions were localized into the electron-density maps of the two structures: one chloride ion at the interface between two symmetry-related molecules and one sodium ion stabilizing the loop Ser60-Leu75. The sodium ion is surrounded by six ligands which form a bipyramid around it at distances of 2.2-2.6 A.
About this Structure
193L is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
High-resolution structure (1.33 A) of a HEW lysozyme tetragonal crystal grown in the APCF apparatus. Data and structural comparison with a crystal grown under microgravity from SpaceHab-01 mission., Vaney MC, Maignan S, Ries-Kautt M, Ducriux A, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):505-17. PMID:15299672
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