1a4t
From Proteopedia
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|PDB= 1a4t |SIZE=350|CAPTION= <scene name='initialview01'>1a4t</scene> | |PDB= 1a4t |SIZE=350|CAPTION= <scene name='initialview01'>1a4t</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a4t OCA], [http://www.ebi.ac.uk/pdbsum/1a4t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a4t RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A4T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1A4T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A4T OCA]. |
==Reference== | ==Reference== | ||
Solution structure of P22 transcriptional antitermination N peptide-boxB RNA complex., Cai Z, Gorin A, Frederick R, Ye X, Hu W, Majumdar A, Kettani A, Patel DJ, Nat Struct Biol. 1998 Mar;5(3):203-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9501914 9501914] | Solution structure of P22 transcriptional antitermination N peptide-boxB RNA complex., Cai Z, Gorin A, Frederick R, Ye X, Hu W, Majumdar A, Kettani A, Patel DJ, Nat Struct Biol. 1998 Mar;5(3):203-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9501914 9501914] | ||
| + | [[Category: Enterobacteria phage p22]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Yersinia phage py54]] | ||
[[Category: Cai, Z.]] | [[Category: Cai, Z.]] | ||
[[Category: Frederick, R.]] | [[Category: Frederick, R.]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:33:22 2008'' |
Revision as of 15:33, 30 March 2008
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF PHAGE P22 N PEPTIDE-BOX B RNA COMPLEX, NMR, 20 STRUCTURES
Overview
We have determined the solution structure of a 15-mer boxB RNA hairpin complexed with a 20-mer basic peptide of the N protein involved in bacteriophage P22 transcriptional antitermination. Complex formation involves adaptive binding with the N peptide adopting a bent alpha-helical conformation that packs tightly through hydrophobic and electrostatic interactions against the major groove face of the boxB RNA hairpin, orienting the open opposite face for potential interactions with host factors and/or RNA polymerase. Four nucleotides in the boxB RNA hairpin pentaloop form a stable GNRA like tetraloop structural scaffold on complex formation, allowing the looped out fifth nucleotide to make extensive hydrophobic contacts with the bound peptide. The guanidinium group of a key arginine is hydrogen-bonded to the guanine in a loop-closing sheared G.A mismatch and to adjacent backbone phosphates. The identified intermolecular contacts account for the consequences of N peptide and boxB RNA mutations on bacteriophage transcriptional antitermination.
About this Structure
1A4T is a Single protein structure of sequence from Enterobacteria phage p22. Full crystallographic information is available from OCA.
Reference
Solution structure of P22 transcriptional antitermination N peptide-boxB RNA complex., Cai Z, Gorin A, Frederick R, Ye X, Hu W, Majumdar A, Kettani A, Patel DJ, Nat Struct Biol. 1998 Mar;5(3):203-12. PMID:9501914
Page seeded by OCA on Sun Mar 30 18:33:22 2008
