This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1a65
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1a65 |SIZE=350|CAPTION= <scene name='initialview01'>1a65</scene>, resolution 2.23Å | |PDB= 1a65 |SIZE=350|CAPTION= <scene name='initialview01'>1a65</scene>, resolution 2.23Å | ||
|SITE= <scene name='pdbsite=T1:Type+1+Cu+Binding+Site'>T1</scene> and <scene name='pdbsite=T3:Type+3+Cu+Binding+Site'>T3</scene> | |SITE= <scene name='pdbsite=T1:Type+1+Cu+Binding+Site'>T1</scene> and <scene name='pdbsite=T3:Type+3+Cu+Binding+Site'>T3</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a65 OCA], [http://www.ebi.ac.uk/pdbsum/1a65 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a65 RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Brzozowski, W.]] | [[Category: Brzozowski, W.]] | ||
[[Category: Ducros, V.]] | [[Category: Ducros, V.]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: GLC]] | ||
| - | [[Category: NAG]] | ||
| - | [[Category: O]] | ||
[[Category: blue multi-copper oxidase]] | [[Category: blue multi-copper oxidase]] | ||
[[Category: glycoprotein]] | [[Category: glycoprotein]] | ||
| Line 36: | Line 35: | ||
[[Category: type-2 copper depleted]] | [[Category: type-2 copper depleted]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:34:08 2008'' |
Revision as of 15:34, 30 March 2008
| |||||||
| , resolution 2.23Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , , , | ||||||
| Activity: | Laccase, with EC number 1.10.3.2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS
Overview
Laccase catalyses the oxidation of a variety of organic substrates coupled to the reduction of oxygen to water. It is widely believed to be the simplest representative of the ubiquitous blue multi-copper oxidase family. Laccase is implicated in a wide spectrum of biological activities and, in particular, plays a key role in morphogenesis, development and lignin metabolism in fungi and plants. The structure of laccase from the fungus Coprinus cinereus has been determined by X-ray crystallography at a resolution of 2.2 A. Laccase is a monomer composed of three cupredoxin-like beta-sandwich domains, similar to that found in ascorbate oxidase. In contrast to ascorbate oxidase, however, the mononuclear type-1 Cu site lacks the axial methionine ligand and so exhibits trigonal planar coordination, consistent with its elevated redox potential. Crucially, the structure is trapped in a Cu depleted form in which the putative type-2 Cu atom is completely absent, but in which the remaining type-1 and type-3 Cu sites display full occupancy. Type-2 Cu depletion has unexpected consequences for the coordination of the remaining type-3 Cu atoms.
About this Structure
1A65 is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.
Reference
Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:9546223
Page seeded by OCA on Sun Mar 30 18:34:08 2008
